Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells

Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells Publisher

Najafi S^{1, 2, 3} ; Nosrati H⁴ ; Faraji Z⁵ ; Mohamadnia A^{6, 7} ; Shirian S^{8, 9, 10} ; Mortazavi SM¹¹ ; Bahrami N^{12, 13}

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1. Dental Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. Dept. of Oral & Maxillofacial Medicine, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
3. Dept of Oral & Maxillofacial Medicine, School of Dentistry, International Campus, Tehran University of Medical Sciences, Tehran, Iran
4. Tehran University of Medical Sciences, Tehran, Iran
5. Tehran University of Medical Science, Tehran, Iran
6. Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), ShahidBeheshti University of Medical Sciences, Tehran, Iran
7. Department of Biotechnology, School of Advanced Technologies in Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran
8. Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
9. Shiraz Molecular Pathology Research Cenetr, Dr Daneshbod Lab, Shiraz, Iran
10. Shefa Neuroscience Reseach Center, Tehran, Iran
11. Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Alborz University of Medical Science, Karaj, Iran
12. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
13. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Heliyon Published:2020

Abstract

Background: The efficacy of mesnchymal stem cells (MSCs) to treat the necrotic tissue of salivary glands (SGs) has yet investigated. Objective: This study was conducted to investigate the potential capacity of MSCs to restore the function and regenerate the necrotic submandiular gland in the rat animal model. Methods: Twenty-one Sprague–Dawley rats were provided from a breeding colony and randomly divided into three groups including the positive control or induced SG atrophy without treatment, the treatment group or induced SG atrophy with MSCs isolated transplantation and the negative control group consists of healthy rats. The atrophic and necrotic submandiular gland was induced using intraoral duct ligation of the main duct of submandiular gland for one month. The isolated stem cells were confirmed using flow cytometry for CD90 and CD 105. The isolated MSCs were cultured and injected to submandiular gland and the potential efficacy of MSCs to treat the atrophic submandibular glands was evaluated using histopathology on two weeks post-transplantation. To detect the acinar cell protein secretory granules, Alcian Blue and periodic acid shift (PAS) staining were done. For the demonstration of mitotic index or proliferation rate of the SG epithelia tissue, Ki-67 and Smbg proteins expression were evaluated using immunohistochemistry. Results: The locally injected MSCs could regenerate the overall histological structure of the necrotic submandibular gland tissue within 2 weeks of post-transplantation. Alcian Blue and PAS staining indicated that the mean amount of serous and mucin secretions in the treatment group was significantly increased compared to the positive control groups. We have also found that the treatment group significantly express higher Ki-67 protein, as a diagnostic marker for cell mitosis and proliferation rate, and lower Smbg protein, as a diagnostic marker, for damage to the submandibular gland than that of control group. Conclusion: This study demonstrates the therapeutic benefits of MSCs isolated from the SG in treating atrophic and necrotic SGs in a rat model. MSCs may be potential candidates for cell-based therapies targeting hypofunction of SG induced by a range of diseases or because of surgery and radiotherapy of head and neck cancers. © 2020; Biochemistry; Cancer research; Cell biology; Molecular biology; Regenerative medicine; Stem cell research; Dry mouth; Salivary glands reconstruction; Stem cell transplantation © 2020

2. Muscarinic Cholinergic Receptors in Minor Salivary Gland Tissues of Patients With Oral Lichen Planus: A Case-Control Study, Journal of Oral Pathology and Medicine (2020)

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Nafiseh Sheykhbahaei (1)

Mahdieh-Sadat Moosavi (1)

Style	Citing Format
MLA	Najafi S, et al.. "Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells." Heliyon, vol. 6, no. 10, 2020, pp. -.
APA	Najafi S, Nosrati H, Faraji Z, Mohamadnia A, Shirian S, Mortazavi SM, Bahrami N (2020). Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells. Heliyon, 6(10), -.
Chicago	Najafi S, Nosrati H, Faraji Z, Mohamadnia A, Shirian S, Mortazavi SM, Bahrami N. "Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells." Heliyon 6, no. 10 (2020): -.
Harvard	Najafi S et al. (2020) 'Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells', Heliyon, 6(10), pp. -.
Vancouver	Najafi S, Nosrati H, Faraji Z, Mohamadnia A, Shirian S, Mortazavi SM, et al.. Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells. Heliyon. 2020;6(10):-.
BibTex	@article{ author = {Najafi S and Nosrati H and Faraji Z and Mohamadnia A and Shirian S and Mortazavi SM and Bahrami N}, title = {Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells}, journal = {Heliyon}, volume = {6}, number = {10}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Najafi S AU - Nosrati H AU - Faraji Z AU - Mohamadnia A AU - Shirian S AU - Mortazavi SM AU - Bahrami N TI - Reconstruction of Necrotic Submandibular Salivary Gland Using Mesenchymal Stem Cells JO - Heliyon VL - 6 IS - 10 SP - EP - PY - 2020 ER -

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