Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Association Between Mutations in Gyra and Parc Genes of Acinetobacter Baumannii Clinical Isolates and Ciprofloxacin Resistance



Ardebili A1 ; Lari AR2 ; Beheshti M3 ; Lari ER4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Microbiology, Golestan University of Medical Sciences, Gorgan, Iran
  2. 2. Department of Microbiology, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Science and Environment, Technology and Communication, Liege, Belgium

Source: Iranian Journal of Basic Medical Sciences Published:2015

Abstract

Objective(s): We investigated the contribution of gyrA and parC mutational mechanism in decreased ciprofloxacin susceptibility of Acinetobacter baumannii isolated from burn wound infections. Materials and Methods: Ciprofloxacin susceptibility of 50 A. baumannii isolates was evaluated by disk diffusion and agar dilution methods. PCR and sequencing were performed for detection of mutation in gyrA and parC genes. Results: The 44 and 4 isolates of A. baumannii exhibited full and intermediate‐resistant to ciprofloxacin, respectively. Overall, the 42 isolates with double mutations of gyrA and parC genes showed a higher level of ciprofloxacin resistance than the 3 isolates with single mutations of gyrA or parC. Conclusion: Simultaneous mutations in gyrA and parC genes are expected to play a major role in highlevel fluoroquinolone resistance in A. baumannii; albeit a single mutation in DNA topoisomerase IV could occasionally be associated with intermediate‐resistance to these antimicrobials. © 2015, Mashhad University of Medical Sciences. All rights reserved.
Related Docs
1. Carbapenem-Resistant Acinetobacter Baumannii Recovered From Burn Patients, Journal of Pharmacy and Pharmaceutical Sciences (2016)
Experts (# of related papers)