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Nitric Oxide Mediates Effects of Acute, Not Chronic, Naltrexone on Lps-Induced Hepatic Encephalopathy in Cirrhotic Rats Publisher Pubmed



Ghiassy B1, 2, 3 ; Rahimi N1, 2, 3 ; Javadipaydar M1, 2, 3 ; Gharedaghi MH1, 2, 3 ; Norouzijavidan A1, 2, 3 ; Dehpour AR1, 2, 3
Authors
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Authors Affiliations
  1. 1. c Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran
  2. 2. c Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran
  3. 3. c Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran

Source: Canadian journal of physiology and pharmacology Published:2017


Abstract

Recent studies suggest endogenous opioids and nitric oxide (NO) are involved in the pathophysiology of hepatic encephalopathy (HE). In this study, the interaction between the opioid receptor antagonist and NO was investigated on lipopolysaccharide (LPS)-induced HE in cirrhotic rats. Male rats were divided in the sham- and bile duct ligation (BDL)-operated groups. Animals were treated with saline; naltrexone (10 mg/kg, i.p.); or L-NAME (3 mg/kg, i.p.), alone or in combination with naltrexone. To induce HE, LPS (1 mg/kg, i.p.) was injected 1 h after the final drug treatment. HE scoring, hepatic histology, and plasma NO metabolites levels and mortality rate were recorded. Deteriorated level of consciousness and mortality after LPS administration significantly ameliorated following both acute and chronic treatment with naltrexone in cirrhotic rats. However, acute and chronic administration of L-NAME did not change HE scores in cirrhotic rats. The effects of acute but not chronic treatment of naltrexone on HE parameters were reversed by L-NAME. Plasma NOx concentrations elevated in BDL rats, which were decreased after acute and chronic treatment by naltrexone or L-NAME, significantly. We suggest both acute and chronic treatment with naltrexone improved LPS-induced HE. But, only acute treatment with naltrexone may affect through NO pathway.