The Comparison of Sensitivity and Specificity of Elisa-Based Microneutralization Test With Hemagglutination Inhibition Test to Evaluate Neutralizing Antibody Against Influenza Virus (H1n1)



Tavakoli A¹ ; Rezaei F¹ ; Fatemi Nasab GS¹ ; Adjaminezhadfard F¹ ; Noroozbabaei Z¹ ; Mokhtariazad T¹ Show Affiliations
Source: Iranian Journal of Public Health Published:2017

Abstract

Background: The most common serological assay to measure anti-influenza antibodies is hemagglutination inhibition (HI) assay. Recently, neutralizing antibodies against influenza virus infection or vaccination can also be detected using microneutralization assays and occasionally, have greater sensitivity than the standard HI assays. The study aimed to compare the sensitivity and specificity of ELISA-based microneutralization (microNT-ELISA) and conventional HI assays in order to detect influenza H1N1 virus antibodies. Methods: MicroNT-ELISA was set up according to the WHO Manual on Influenza Diagnosis and Surveillance in Virology Department of Tehran University of Medical Sciences for the detection of neutralizing antibodies against H1N1 influenza virus in 2013. Fifty serum samples were analyzed with both HI and microNT-ELISA assays. Correla-tion between methods was calculated by linear regression analysis. Results: The linear correlation coefficient squares, R2, of microNT-ELISA and HI test was 0.61 (P<0.0001) and we observed a high index of coincidence between the two tests. According to McNemar’s test, there was no statistically significant difference between these two assays (P>0.05). Conclusion: The sensitivity and specificity of microNT-ELISA assay were high (87% and 73%, respectively) and closely related to gold standard test results. Therefore, microNT-ELISA is recommended as an alternative or comple-mentary test to conventional HI assay for serological and epidemiological purposes. © 2017, Iranian Journal of Public Health. All rights reserved.