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Alpha-Lipoic Acid and Coenzyme Q10 Combination Ameliorates Experimental Diabetic Neuropathy by Modulating Oxidative Stress and Apoptosis Publisher Pubmed



Sadeghiyan Galeshkalami N1 ; Abdollahi M2, 3 ; Najafi R4 ; Baeeri M2 ; Jamshidzade A5 ; Falak R6 ; Davoodzadeh Gholami M6 ; Hassanzadeh G7 ; Mokhtari T8, 9 ; Hassani S2 ; Rahimifard M2 ; Hosseini A10
Authors
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Authors Affiliations
  1. 1. Department of Pharmacology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
  2. 2. Toxicology and Diseases Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Science, Tehran, Iran
  3. 3. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  5. 5. Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  6. 6. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran
  9. 9. Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  10. 10. Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran

Source: Life Sciences Published:2019


Abstract

Aims: Diabetic neuropathy (DN) is the most common complication of diabetes. Neuroprotective effects of alpha lipoic acid (ALA) and coenzyme Q10 (CoQ10) has been previously shown in DN, but underlying mechanisms involved not been exactly found. The present study explored the neuroprotective effects of ALA and Q10 combination in experimental DN by ameliorating oxidative stress and apoptosis. Main methods: We investigated the effects of CoQ10 (10 mg/kg, orally, five weeks) and/or ALA (100 mg/kg, orally, five weeks) in STZ (45 mg/kg, i.p.)- induced DN in rats. After treatments motor function, oxidative stress biomarkers, ATP levels, expression of caspase 3 and UCP2 proteins were assessed by open-field, biochemical and ELISA methods and Western blot analysis. Dorsal root ganglion (DRG) neurons were histologically examined using H&E staining method. Key findings: ALA and/or CoQ10 treatment significantly (p < 0.05) attenuated DN – induced motor function deficiency by modulating distance moved and velocity. ALA and/or CoQ10 treatment dramatically suppressed DN – induced oxidative stress which was associated with decrease in LPO and ROS and increase in GSH and TAC in DRG neurons. ALA and/or CoQ10 was proved to prevent apoptosis and degeneration of DRG neurons, which appears to be mediated by regulating the expression of caspase 3 and UCP2 proteins, inducing ATP and improving DN–induced changes in DRG neurons. We found maximum effectiveness with ALA and CoQ10 combination on mentioned factors. Significance: These results provide a possible basis of the underlying mechanism for application of ALA and CoQ10 combination in treatment of DN. © 2018 Elsevier Inc.
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