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Investigation of Human Parvovirus B19 Prevalence in a Large Healthy Umbilical Cord Blood Donors Publisher



Ramezany H1 ; Kheirandish M1 ; Samiee S1 ; Khosravifar M2 ; Hashemi SM2
Authors
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Authors Affiliations
  1. 1. Department of Immunology, Research Center of Blood Transfusion, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
  2. 2. Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Microbiology Published:2022


Abstract

Background and Objectives: Umbilical cord blood (UCB) was used to source hematopoietic stem cells in the past. Despite the apparent advantages of UCB transplantation, virus reactivation poses a considerable danger in allogeneic hematopoietic stem cell transplantation (HSCT). Human Parvovirus B19 is regarded as a potential threat to UCB contamination. This study aimed to evaluate the prevalence of parvovirus B19 in cord blood donors by Semi-Nested PCR. This study is the first largescale report of the B19 DNA in cord blood donors in Iran. Materials and Methods: A total of 691 umbilical cord blood were collected under standard procedure. Then, DNA from buffy coat and plasma were extracted, and semi-nested PCR was performed for all samples. Results: Two out of 691 samples (0.29%) indicated viremia in plasma and buffy coat. Conclusion: In this line, designing and validating a quantitative PCR assay for detection, quantification, and discrimination of Human B19 DNA genotypes of cord blood donors is necessary to enhance the safety of this source of stem cells. © 2022 The Authors. Published by Tehran University of Medical Sciences.
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