Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 2 Discriminates Ags and Mkn45 Cells of Human Gastric Cancer Publisher



Rezaeitavirani M ; Razi F ; Bandarian F ; Robati RM ; Rezaei M ; Naderi N
Source: Gastroenterology and Hepatology from Bed to Bench Published:2025

Abstract

Aim: In the present study, differences between these cell lines are discovered via gene expression analysis. Background: Gastric cancer is a foremost health threat worldwide with more than 650000 deaths annually. The MKN 45 cell line (the ordinary gastric mucosa) is originated from undifferentiated carcinomas, while the AGS cells display epithelial morphology and is derived from gastric adenocarcinoma. Methods: The significantly differentially expressed genes (DEGs) from the Gene Expression Omnibus (GEO) database, which discriminate AGS and MKN45 cells in the presence of DMSO and verteporfin (a chemotherapy reagent), are determined. The key DEGs were identified based on linear regression analysis and enriched to find the cellular compartment, bulk tissue gene expression, and pathways via GeneCards database. Kaplan-Meier Plot was applied to explore the percentage survival of gastric cancer patients based on the expression level of the introduced key genes. Results: About 12000 significant DEGs discriminate AGS and MKN45 cells. Procollagen-Lysine,2-oxoglutarate 5-dioxygenase 2 (PLOD2) was highlighted as the key gene among the studied significant DEGs. Collagen chain trimerization and extracellular matrix organization super-pathways, as related pathways and extracellular space and endoplasmic reticulum as the main cellular compartments were identified as the involved parts. Conclusion: MKN45 was highlighted as more invasive cancer cell relative to AGS cells. PLOD2 was pointed out as a suitable target in the chemotherapy of gastric cancer. Copyright © 2025, Gastroenterology and Hepatology From Bed to Bench (GHFBB). This is an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits others to copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.