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Modulation of the Immune Response to Dna Vaccine Encoding Gene of 8-Kda Subunit of Echinococcus Granulosus Antigen B Using Murine Interleukin-12 Plasmid in Balb/C Mice



Azizi H1, 2 ; Kazemi B3, 4 ; Bandehpour M4 ; Mohebali M1, 5 ; Khamesipour A6 ; Aryaeipour M2 ; Yaghoobi H4 ; Rokni MB2, 5
Authors
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Authors Affiliations
  1. 1. Dept. of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran
  2. 2. Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Dept. of Medical Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Skin and Leprosy Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Parasitology Published:2016

Abstract

Background: The current study was designed to evaluate immune responses induced by DNA vaccines encoding 8-kDa subunit of antigen B (HydI) of Echinococcus granulosus and murine interleukin 12 (IL-12) as genetic adjuvants in BALB/c mice. Methods: Expression plasmid pcDNA3.1 containing HydI (pcHyd1) as vaccine along with the murine interleukin 12 (pcMIL12) as adjuvant were used. Thirty-five mice in the five experimental groups received PBS, empty pcDNA3.1, pcHydІ, pcMIL-12, and pcHydІ+ pcMIL-12 in days zero, 14th and 28th. Two weeks after the last immunization, evaluation of the immune response was performed by evaluating the proliferation of splenic lymphocytes, IFN-γ and IL-4, determination of IgG isotyping titer. Results: Mice that received the pcHydI+pcMIL12 exhibited higher levels of lymphocyte proliferation compared to mice that received the pcHydI alone (P<0.001), and produced significantly more IFN-γ in comparison to other groups (P< 0.001). In addi-tion, they produced significantly less IL-4 than mice receiving the PBS and the empty plasmid (P<0.023). The IgG2a levels were clearly higher in pcHydI+pcMIL12 group in comparison with the groups of pcHydI alone, empty plasmid, and PBS. In contrast, IgG1 was elevated in the group of pcHydI. Conclusion: Co-delivery of IL-12 with DNA encoding 8-kDa subunit of antigen B was effective significantly in inducing the immune response in mice. © 2016, Tehran University of Medical Sciences (TUMS). All rights reserved.