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A Highly Sensitive Electrochemical Biosensor Based on Aunp-Modified Gold Electrodes for Selective Determination of Serum Levels of Crosslaps Publisher



Khashayar P1, 2, 3 ; Amoabediny G4, 5 ; Larijani B6 ; Hosseini M1 ; Verplancke R2 ; De Keersmaecker M7 ; Adriaens A7 ; Goemaere S8 ; Fiers T8 ; Vanfleteren J2
Authors

Source: 3 Biotech Published:2017


Abstract

This article explains a step-wise protocol to develop an electrochemical sensor to quantify serum levels of C-telopeptide (CTX) crosslinks also known as crosslaps in a matter of minutes and with high level of accuracy. The technique needs only one-step (incubation) and can thus be used for point of care screening. Due to the excellent electrical properties of the as-prepared immunosensor, CTX levels were successfully measured from 1 to 1000 pg/mL. This is while the normal reference of the marker is 50–450 pg/mL, suggesting that the sensor can acceptably detect CTX. The results also showed a good correlation with ECLIA in measuring serum levels of CTX. © 2017, Springer-Verlag GmbH Germany.