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Preliminary Information of Iranian Lizard Leishmania Promastigote Transcriptome Sequencing by Next-Generation Sequencing (Ngs) Method



Ehya F1 ; Kalantari S2, 3 ; Bandehpour M4, 5 ; Kazemi B4, 5
Authors
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Authors Affiliations
  1. 1. Department of Biotechnology, Tarbiat Modares University, Tehran, Iran
  2. 2. Digestive Oncology Research Center, Digestive Diseases Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Regenerative Medicine Group (REMED), Universal Scientific Education & Research Network (USERN), Tehran, Iran
  4. 4. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Parasitology Published:2023

Abstract

Background: A lizard Leishmania has been isolated from a lizard (Agama agilis) in Iran. Its genome sequence has not been determined, so far. Methods: The study was done at Shahid Beheshti University of Medical Sciences, Tehran, Iran in 2017-2023. Leishmania promastigotes were cultured in RPMI1640 culture medium and collected at logarithmic phase by centrigugation. Parasite RNA was extracted by the Qiagene standard kit and its quantity and quality was determined and sequenced by NGS method with Illumina PE machine at BGI Company (China). Results: The number of 8316 mRNA, 83 tRNA, 63 rRNA, 83 ncRNA, 5 snRNA, 1039 snoRNA, 36 region, and 3 repeat regions, 8343 CDS, 9597 Exon and 9292 Genes were identified in promastigote of Iranian lizard Leishmania. Conclusion: Genomic elements of Iranian lizards Leishmania (with unique characteristics) were determined and identified by NGS system. © 2023 Ehya et al. Published by Tehran University of Medical Sciences.
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