Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Role of Progesterone in Cellular Apoptosis of Skin and Lung in a Bleomycin-Injured Mouse Model Publisher Pubmed



Vafashoar F1 ; Poormoghim H2 ; Mousavizadeh K3 ; Shabestari TM4 ; Tavasoli A5 ; Javadmoosavi SA6 ; Mojtabavi N1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Immunology, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Scleroderma Study Group, Firoozgar Hospital, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Institute of Immunology and Infectious Disease, Antimicrobial Resistance Research Center, Tehran, Iran
  5. 5. Department of Pathobiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  6. 6. Air Pollution Research Centre, School of Public Health, Iran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Allergy# Asthma and Immunology Published:2019


Abstract

Systemic sclerosis is a female predominant, a fibrotic autoimmune disease in which disturbance in tissue homeostasis and cell turnover including cell apoptosis are central events in pathogenesis. Sex hormones are known as the important players in sexual dimorphism of autoimmune diseases and in tissue homeostasis. Progesterone influences autoimmune disease via its immunomodulatory effect or by its direct action on parenchymal cell function. On the other hand, this hormone impacts tissue homeostasis by acting on cell apoptosis in a different situation. The objective of this study was to examine the effect of progesterone on cellular apoptosis of skin and lung tissues in a mouse model of scleroderma. Four group of mice were involved in this study with 10 mice in each. The fibrotic model was induced by daily subcutaneous injection of bleomycin for 28 days. One week after initiation of fibrosis induction, mice received subcutaneous progesterone alone or with bleomycin for 21 days. Control group received only Phosphate buffered saline PBS. After 28 days, under lethal anesthesia skin and lung tissues were harvested for histological assessment and hydroxyproline measurement. Apoptosis in tissue sections was detected by TUNEL assay technique. Bleomycin administration induced fibrosis in skin and lung tissues. Severe apoptosis was seen in skin and lung tissues of the bleomycin-treated group (p<0.001 in the skin and p<0.05 in the lung). Progesterone injection either in the skin (p>0.05) or in the lung (p>0.05) did not alter apoptosis in bleomycin-treated animals. Our data confirm the role of apoptosis in the pathogenesis of fibrosis in this model; however, progesterone does not affect cellular apoptosis in skin and lung tissues of bleomycin-injured animals. Copyright© February 2019, Iran J Allergy Asthma Immunol. All rights reserved.
Related Docs
1. Natural Formulations: Novel Viewpoint for Scleroderma Adjunct Treatment, Journal of Immunology Research (2021)
2. Co-Administration of Progesterone and Melatonin Attenuates Ischemia-Induced Hippocampal Damage in Rats, Journal of Molecular Neuroscience (2018)
3. C-Abl Silencing Reduced the Inhibitory Effects of Tgf-Β1 on Apoptosis in Systemic Sclerosis Dermal Fibroblasts, Molecular and Cellular Biochemistry (2015)
4. Gelatinases Increase in Bleomycin-Induced Systemic Sclerosis Mouse Model, Iranian Journal of Allergy# Asthma and Immunology (2019)
5. Inhibition of Microrna-21 Induces Apoptosis in Dermal Fibroblasts of Patients With Systemic Sclerosis, International Journal of Dermatology (2016)
Experts (# of related papers)
View all Related Experts
Ahmad Reza Jamshidi (2)
Mahdi Mahmoudi (2)