Comparison of a New In-House Hiv-1 Taqman Real-Time Pcr and Three Commercial Hiv-1 Rna Quantitative Assays Publisher Pubmed



Noorbazargan H¹ ; Nadji SA² ; Mirab Samiee S³ ; Paryan M^{4, 7} ; Mohammadiyeganeh S^{5, 6}
Source: Comparative Immunology# Microbiology and Infectious Diseases Published:2018

Abstract

Objectives: The aim of this study was to compare the analytical performance of an In-House HIV-1 viral load determination technique with three commercial kits including COBAS® AmpliPrep, RealStar®, and RTA® HIV-1 Real-Time PCR. Results: A total of 100 HIV-1 suspicious plasma samples were tested by the In-House TaqMan® Real-Time PCR assay along with the above-mentioned kits. Comparative analysis between In-House and reference method (COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test version 2.0) showed high concordance with a mean difference of 0.08 log10 copies/ml. All samples results were within -0.16–0.31 log10 copies/ml. A suitable correlation was obtained with a coefficient (R2) of 0.82 between the In-House assay and RTA® Kit, however, two positive samples were not detected. The lowest agreement was detected with RealStar® HIV Kit 1.0 (R2 = 0.49, r = 0.7). Conclusions: The newly developed method has suitable sensitivity, accuracy, and precision. In addition, it is cost-effective and can be an alternative in all laboratories. © 2018 Elsevier Ltd