Comparison of Tgf-Β1 and No Production by Mesenchymal Stem Cells Isolated From Murine Lung and Adipose Tissues Publisher Pubmed



Hosseinpur Z¹ ; Hashemi SM² ; Salehi E³ ; Ghazanfari T¹ Show Affiliations
Source: Immunopharmacology and Immunotoxicology Published:2016

Abstract

Abstract: Context: Mesenchymal stem cells (MSCs) are cell sources for tissues regeneration. By secretion of soluble factors including transforming growth factor-β (TGF-β1) and nitric oxide (NO), MSCs are also able to regulate the immune system. MSCs have been disclosed in lung and adipose tissues with insufficient comparison between the tissues. Objectives: In this study, specific differentiation and the expression of surface antigens as well as TGF-β1 and NO productive levels were compared in murine lung-derived MSCs (LMSCs) and adipose tissue-derived MSCs (ADMSCs). Materials and methods: MSCs were isolated from murine lung and adipose tissues and cultured. Both cell populations were characterized using multilineage potential and the expression of surface antigenic proteins, CD73, CD105, CD34, CD45, and CD11b. Finally, levels of TGF-β1 and NO were evaluated and compared in ADMSCs and LMSCs. Results: Expression of CD73 and CD105; lack of the expression of CD34, CD45, and CD11b markers; as well as adipocyte and osteocyte differentiations were detected in both adult stem cells. No significant difference was found in TGF-β1 and NO production between two stem cell populations. Conclusion: Our data showed that LMSCs and ADMSCs have comparable phenotype and TGF-β1 and NO production. © 2016 Informa UK Limited, trading as Taylor & Francis Group.