Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Association Between Wolbachia Infection and Susceptibility to Deltamethrin Insecticide in Phlebotomus Papatasi (Diptera: Psychodidae), the Main Vector of Zoonotic Cutaneous Leishmaniasis Publisher Pubmed



Azarm A1, 2 ; Koosha M1 ; Dalimi A2 ; Zahraieramazani A1 ; Akhavan AA1 ; Saeidi Z1 ; Mohebali M3 ; Azam K4 ; Vatandoost H1, 5 ; Oshaghi MA1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Parasitology and Entomology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Chemical Pollutants and Pesticides, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran

Source: Vector-Borne and Zoonotic Diseases Published:2024


Abstract

Background: Phlebotomus papatasi (Diptera: Psychodidae) is the main vector of zoonotic cutaneous leishmaniasis. Wolbachia is a symbiotic alphaproteobacteria of arthropods that can be involved in susceptibility or resistance. This study aimed to investigate the relationship between Wolbachia and Deltamethrin susceptibility/ resistance in Ph. papatasi. Deltamethrin filter papers (0.00002%) were used to test sand fly field collected from southern Iran. After the test, PCR amplification of the Wolbachia surface protein gene (wsp) was used to measure Wolbachia infection rate in the killed, surviving, and control groups. Result: The rates of infection by Wolbachia strain (wPap, super group A) differed between killed (susceptible) and surviving (resistant) Ph. papatasi specimens. The rate of Wolbachia infection in susceptible individuals was more than twice (2.3) (39% vs. 17%) in resistant individuals with the same genetic background. This difference was highly significant (p < 0.001), indicating a positive association between Wolbachia infection and susceptibility to Deltamethrin. In addition, the results showed that Deltamethrin can act as a PCR inhibitor during detection of Wolbachia in Ph. papatasi. Conclusion: Results of this study show that Wolbachia is associated with Deltamethrin susceptibility level in Ph. papatasi. Also, as Deltamethrin has been identified as a PCR inhibitor, great care must be taken in interpreting Wolbachia infection status in infected populations. The results of this study may provide information for a better understanding of the host-symbiont relationship, as well as application of host symbiosis in pest management. © Mary Ann Liebert, Inc.
Related Docs
View other Related Docs
1. Relationship Between Wolbachia Infection in Culex Quinquefasciatus and Its Resistance to Insecticide, Heliyon (2021)
2. Wsp-Based Analysis of Wolbachia Strains Associated With Phlebotomus Papatasi and P. Sergenti (Diptera: Psychodidae) Main Cutaneous Leishmaniasis Vectors, Introduction of a New Subgroup Wserg, Pathogens and Global Health (2018)
3. A Survey of Wolbachia Infection in Brachyceran Flies From Iran, PLoS ONE (2024)
4. Wolbachia Infection in Native Populations of Blattella Germanica and Periplaneta Americana, PLoS ONE (2023)
5. Wolbachia Infection in West Nile Virus Vectors of Northwest Iran, Applied Entomology and Zoology (2020)
6. The Role of Midgut Symbiotic Bacteria in Resistance of Anopheles Stephensi (Diptera: Culicidae) to Organophosphate Insecticides, Pathogens and Global Health (2017)
7. Leishmania Spp Infection in Patients and Great Gerbils (Rhombomys Opimus) in a High-Risk Focus of Zoonotic Cutaneous Leishmaniasis in Central Iran: A Microscopic and Molecular Survey, Journal of Arthropod-Borne Diseases (2025)
8. Rodents As Vehicle for Delivery of Transgenic Bacteria to Make Paratransgenic Sand Fly Vectors of Cutaneous Leishmaniasis in Field Condition, Scientific Reports (2023)
Experts (# of related papers)
View all Related Experts
Mohammad Ali Oshaghi (15)
Hassan Vatandoost (7)
Other Related Docs
9. Wolbachia Endobacteria in Natural Populations of Culex Pipiens of Iran and Its Phylogenetic Congruence, Journal of Arthropod-Borne Diseases (2016)
10. Molecular Detection of Dirofilaria Spp. and Host Blood-Meal Identification in the Simulium Turgaicum Complex (Diptera: Simuliidae) in the Aras River Basin, Northwestern Iran, Parasites and Vectors (2020)
11. Bioactivities of Rose-Scented Geranium Nanoemulsions Against the Larvae of Anopheles Stephensi and Their Gut Bacteria, PLoS ONE (2021)
12. Utility of Mtdna-Coi Barcode Region for Phylogenetic Relationship and Diagnosis of Five Common Pest Cockroaches, Journal of Arthropod-Borne Diseases (2017)
13. Evaluation of the Entomopathogenic Fungus Beauveria Bassiana on Different Stages of Phlebotomus Papatasi (Diptera: Psychodidae), Vector of Zoonotic Cutaneous Leishmaniasis in Iran, Journal of Arthropod-Borne Diseases (2023)
14. Molecular and Biochemical Detection of Insecticide Resistance in the Leishmania Vector, Phlebotomus Papatasi (Diptera: Psychodidae) to Dichlorodiphenyltrichloroethane and Pyrethroids, in Central Iran, Journal of Medical Entomology (2022)
15. The Structure and Diversity of Microbial Communities in Paederus Fuscipes (Coleoptera: Staphylinidae): From Ecological Paradigm to Pathobiome, Microbiome (2023)
16. Alternative Strategies for Mosquito-Borne Arbovirus Control, PLoS Neglected Tropical Diseases (2019)
17. Identification of Forensically Important Flesh Flies Using the Cytochrome C Oxidase Subunits I and Ii Genes, Journal of Medical Entomology (2019)
18. Comparative Analysis of the Gut Microbiota of Sand Fly Vectors of Zoonotic Visceral Leishmaniasis (Zvl) in Iran; Host-Environment Interplay Shapes Diversity, PLoS Neglected Tropical Diseases (2022)
19. Treatment Failure in Cutaneous Leishmaniasis Patients Referred to the School of Public Health, Tehran University of Medical Sciences During 2008-2017, Journal of Arthropod-Borne Diseases (2020)
20. Aerobic Bacterial Flora of Biotic and Abiotic Compartments of a Hyperendemic Zoonotic Cutaneous Leishmaniasis (Zcl) Focus, Parasites and Vectors (2015)
21. Leishmania Spp. Infection in Rhombomys Opimus and Meriones Libycus As Main Reservoirs of Zoonotic Cutaneous Leishmaniasis in Central Parts of Iran: Progress and Implications in Health Policy, Acta Tropica (2022)