Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis

Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis Publisher Pubmed

Shomali N^{1, 2, 3} ; Baradaran B^{1, 2} ; Daei Sorkhabi A³ ; Sarkesh A³ ; Saeed Kahrizi M⁴ ; Tosan F⁵ ; Mahmoodpoor A⁶ ; Mardi A⁷ ; Mohammadi H^{8, 9} ; Hassanzadeh A¹⁰ ; Saeedi H¹ ; Hajialilo M¹¹ ; Hemmatzadeh M² ; Marofi F¹ Show All Authors

Shomali N^{1, 2, 3}
Baradaran B^{1, 2}
Daei Sorkhabi A³
Sarkesh A³
Saeed Kahrizi M⁴
Tosan F⁵
Mahmoodpoor A⁶
Mardi A⁷
Mohammadi H^{8, 9}
Hassanzadeh A¹⁰
Saeedi H¹
Hajialilo M¹¹
Hemmatzadeh M²
Marofi F¹
Sandoghchian Shotorbani S^{1, 2}

Show Affiliations

1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2. Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
4. Department of Surgery, Alborz University of Medical Sciences, Karaj, Iran
5. Student Research Committee, Semnan University of Medical Sciences, Semnan, Iran
6. Department of Anesthesiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
7. Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
8. Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran
9. Department of Immunology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
10. Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
11. Connective Tissue Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Gene Published:2022

Abstract

Ankylosing spondylitis (AS) is progressive immune-mediated arthritis. Persistent autoreactivity of T cells with an up-regulated Survivin expression is strongly implicated in AS immunopathogenesis. Besides, Survivin can inhibit proapoptotic caspase 9 activations. Moreover, microRNAs are small non-coding RNAs that are dysregulated in various diseases, in which their altered expression could modulate Survivin expression. The primary goal of this study was to assess the role of Survivin and its-targeting microRNAs in the immunopathogenesis of AS disease. For this aim, peripheral blood mononuclear cells (PBMCs) were isolated from 15 patients with AS and healthy matched controls using Ficoll-Hypaque. T cells were obtained using the magnetic-activated cell sorting (MACS) method. After that, the expression levels of Survivin, Caspase 9, and specific miRNAs were determined using qT-qPCR. Also, the expression of Survivin and Caspase 9 at protein levels was determined by western blotting. Then, the isolated T cells were co-cultured with interleukin (IL)-2 and muromonab-CD3 (OKT-3) for active-induced cell death (AICD) induction, Survivin siRNA for inhibition of Survivin expression, and their combination to assess the implication of Survivin expression in autoreactive T lymphocytes' resistance to apoptosis by determining the rate of apoptosis by Flowcytometry assay. The results showed that Survivin was up-regulated while Caspase 9 was downregulated in patients with AS. It was also revealed that microRNAs that directly or indirectly target the Survivin mRNA were dysregulated in patients with AS. It was also revealed that T cells obtained from AS patients were more resistant to apoptosis induction than those obtained from healthy people. In summary, the results obtained from this study showed that dysregulation of Survivin and Survivin-targeting miRNAs in T lymphocytes obtained from AS patients contribute to their resistance to apoptosis, suggesting the future development of targeted therapies for AS. © 2022

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Style	Citing Format
MLA	Shomali N, et al.. "Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis." Gene, vol. 844, no. , 2022, pp. -.
APA	Shomali N, Baradaran B, Daei Sorkhabi A, Sarkesh A, Saeed Kahrizi M, Tosan F, Mahmoodpoor A, Mardi A, Mohammadi H, Hassanzadeh A, Saeedi H, Hajialilo M, Hemmatzadeh M, Marofi F, Sandoghchian Shotorbani S (2022). Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis. Gene, 844(), -.
Chicago	Shomali N, Baradaran B, Daei Sorkhabi A, Sarkesh A, Saeed Kahrizi M, Tosan F, Mahmoodpoor A, et al.. "Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis." Gene 844, no. (2022): -.
Harvard	Shomali N et al. (2022) 'Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis', Gene, 844(), pp. -.
Vancouver	Shomali N, Baradaran B, Daei Sorkhabi A, Sarkesh A, Saeed Kahrizi M, Tosan F, et al.. Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis. Gene. 2022;844():-.
BibTex	@article{ author = {Shomali N and Baradaran B and Daei Sorkhabi A and Sarkesh A and Saeed Kahrizi M and Tosan F and Mahmoodpoor A and Mardi A and Mohammadi H and Hassanzadeh A and Saeedi H and Hajialilo M and Hemmatzadeh M and Marofi F and Sandoghchian Shotorbani S}, title = {Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis}, journal = {Gene}, volume = {844}, number = {}, pages = {-}, year = {2022} }
RIS	TY - JOUR AU - Shomali N AU - Baradaran B AU - Daei Sorkhabi A AU - Sarkesh A AU - Saeed Kahrizi M AU - Tosan F AU - Mahmoodpoor A AU - Mardi A AU - Mohammadi H AU - Hassanzadeh A AU - Saeedi H AU - Hajialilo M AU - Hemmatzadeh M AU - Marofi F AU - Sandoghchian Shotorbani S TI - Survivin; a Novel Therapeutic Target That Correlates With Survival of Autoreactive T Lymphocytes Obtained From Patients With Ankylosing Spondylitis JO - Gene VL - 844 IS - SP - EP - PY - 2022 ER -

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