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A More Positive Culture by Resin-Containing Media Usage After Suspicious Arthroscopic Infections in Patients Receiving Antimicrobial Therapy Publisher



Bourbour S1 ; Emaneini M1 ; Javad Mortazavi SM2 ; Tahmasebi MN3 ; Yahyazadeh H4 ; Jabalameli M4 ; Jabalameli F1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Tehran University of Medical Sciences, knee and hip surgeon, Department of Orthopedic Surgery, Imam Khomaini Hospital, Tehran, Iran
  3. 3. Tehran University of Medical Sciences, knee surgeon, Department of Orthopedic Surgery, Shariati Hospital, Tehran, Iran
  4. 4. Orthopaedic surgeon, bone and joint reconstruction research center, University of Medical Sciences, Department of Orthopedic Surgery, Shafa Yahyaiyan Hospital, Tehran, Iran

Source: Archives of Bone and Joint Surgery Published:2021


Abstract

Background: Although infections following arthroscopic surgery of cruciate ligament and meniscus are uncommon, they have potentially serious consequences for the graft and articular cartilage. This study aimed to investigate the efficacy of correct sampling and appropriate media, especially resin-containing media, for the detection of infections in patients receiving antibiotics under suspicion of joint infection after arthroscopic anterior cruciate ligament (ACL) and meniscal surgery. In such cases, proper sampling and the use of suitable culture media that cause the neutralization of antibiotics are very effective in isolating microorganisms from the patient samples and positive cultures. Methods: In total, 10 patients who had received antibiotics with suspected knee infection after arthroscopic ACL and meniscal surgery were identified after referral to surgeons during a period of 10 months and investigated in this study. The sample collection, culture on various media (i.e., resin-containing culture media), microbiological tests, and antibiotic susceptibility tests were performed in this study. The amplification of the mecA gene using PCR assay was accomplished for methicillin-resistant staphylococcus strains. Results: This study was conducted on 10 patients who underwent arthroscopic procedures and had received antibiotics. Overall, joint fluid and tissue culture were positive in 60% of the patients. The resin-containing media revealed a trend toward increased detection of bacteria. Coagulase-negative staphylococcus strains were the most frequently isolated bacteria in arthroscopic ACL surgery infections. Out of five methicillin-resistant staphylococcus strains, four strains were found that were resistant to cefoxitin and positive-mecA designated as methicillin-resistant strains. Except for one case, the rest of the staphylococcal strains were resistant to methicillin but susceptible to vancomycin. Conclusion: Despite uncommon and low percentage of infections after arthroscopic ACL and meniscal surgery, the results of our study showed that correct sampling, appropriate cultures, especially aerobic and anaerobic resin-containing media, and microbiological testing remained useful and valuable for diagnosing bacterial infections. © 2021 Mashhad University of Medical Sciences. All rights reserved.