Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells

Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells Publisher

Amjadi F^{1, 2} ; Zandieh Z^{1, 2} ; Salehi E^{2, 3} ; Jafari R⁴ ; Ghasemi N⁵ ; Aflatoonian A⁵ ; Fazeli A⁶ ; Aflatoonian R²

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1. Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2. Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
3. Department of Anatomy, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Immunology, Iran University of Medical Sciences, Tehran, Iran
5. Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
6. Academic Unit of Reproductive and Developmental Medicine, Department of Oncology and Metabolism, University of Sheffield, Sheffield, United Kingdom

Source: Clinical and Experimental Reproductive Medicine Published:2018

Abstract

Objective: To determine the localization, expression, and function of Toll-like receptors (TLRs) in fallopian tube epithelial cells. Methods: The localization of TLRs in fallopian tube epithelial cells was investigated by immunostaining. Surprisingly, the intensity of staining was not equal in the secretory and ciliated cells. After primary cell culture of fallopian tube epithelial cells, ring cloning was used to isolate colonies of ciliated epithelial cells, distinct from non-ciliated epithelial cells. The expression of TLRs 1-10 was examined by quantitative real-time polymerase chain reaction, and protein localization was confirmed by immunostaining. The function of the TLRs was determined by interleukin (IL)-6 and IL-8 production in response to TLR2, TLR3, TLR5, TLR7, and TLR9 ligands. Results: Fallopian tube epithelial cells expressed TLRs 1-10 in a cell-type-specific manner. Exposing fallopian tube epithelial cells to TLR2, TLR3, TLR5, TLR7, and TLR9 agonists induced the secretion of proinflammatory cytokines such as IL-6 and IL-8. Conclusion: Our findings suggest that TLR expression in the fallopian tubes is cell-type-specific. According to our results, ciliated cells may play more effective role than non-ciliated cells in the innate immune defense of the fallopian tubes, and in interactions with gametes and embryos. © 2018. The Korean Society For Reproductive Medicine.

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Style	Citing Format
MLA	Amjadi F, et al.. "Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells." Clinical and Experimental Reproductive Medicine, vol. 45, no. 1, 2018, pp. 1-9.
APA	Amjadi F, Zandieh Z, Salehi E, Jafari R, Ghasemi N, Aflatoonian A, Fazeli A, Aflatoonian R (2018). Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells. Clinical and Experimental Reproductive Medicine, 45(1), 1-9.
Chicago	Amjadi F, Zandieh Z, Salehi E, Jafari R, Ghasemi N, Aflatoonian A, Fazeli A, Aflatoonian R. "Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells." Clinical and Experimental Reproductive Medicine 45, no. 1 (2018): 1-9.
Harvard	Amjadi F et al. (2018) 'Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells', Clinical and Experimental Reproductive Medicine, 45(1), pp. 1-9.
Vancouver	Amjadi F, Zandieh Z, Salehi E, Jafari R, Ghasemi N, Aflatoonian A, et al.. Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells. Clinical and Experimental Reproductive Medicine. 2018;45(1):1-9.
BibTex	@article{ author = {Amjadi F and Zandieh Z and Salehi E and Jafari R and Ghasemi N and Aflatoonian A and Fazeli A and Aflatoonian R}, title = {Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells}, journal = {Clinical and Experimental Reproductive Medicine}, volume = {45}, number = {1}, pages = {1-9}, year = {2018} }
RIS	TY - JOUR AU - Amjadi F AU - Zandieh Z AU - Salehi E AU - Jafari R AU - Ghasemi N AU - Aflatoonian A AU - Fazeli A AU - Aflatoonian R TI - Variable Localization of Toll-Like Receptors in Human Fallopian Tube Epithelial Cells JO - Clinical and Experimental Reproductive Medicine VL - 45 IS - 1 SP - 1 EP - 9 PY - 2018 ER -

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