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Carbonized Aerogel/Zno-Based Dispersive Solid Phase Extraction of Daclatasvir and Sofosbuvir From Biological Samples Prior to Liquid Chromatography–Tandem Mass Spectrometry Publisher Pubmed



Marzi Khosrowshahi E1 ; Hassanpour Sabet R1, 2 ; Afshar Mogaddam MR1 ; Khoubnasabjafari M3, 4 ; Jouybangharamaleki V5 ; Rayatpisheh M6 ; Anushiravani A6 ; Ghanbari R7 ; Jouyban A8, 9
Authors
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Authors Affiliations
  1. 1. Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Department of Anesthesiology and Intensive Care, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  5. 5. Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Gene Therapy Research Center, Digestive Diseases Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  8. 8. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
  9. 9. Faculty of Pharmacy, Near East University, PO BOX: 99138 Nicosia, North Cyprus, Mersin 10, Turkey

Source: Journal of Pharmaceutical and Biomedical Analysis Published:2024


Abstract

Daclatasvir and sofosbuvir are antiviral medications utilized in the treatment of chronic hepatitis C virus (HCV) infection. Due to their low therapeutic index, careful monitoring is necessary to ensure that the optimal dose is administered. High-performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) is an exceptionally sensitive and specific technique for quantifying these drugs within biological matrices. In this study, we developed a novel dispersive solid-phase extraction method employing a carbonized bio aerogel composite with ZnO for efficient extraction of daclatasvir and sofosbuvir from exhaled breath condensate, urine, and plasma samples. The extracted analytes were subsequently subjected to analysis using HPLC–MS/MS. Optimal parameters including pH adjustment, sorbent quantity variation, and elution solvent selection were fine-tuned to achieve maximum recovery efficiency while ensuring selectivity enhancements. The developed method demonstrated broad linearity ranging between 1.2 and 200 ng/mL along with good precision (relative standard deviations ≤6.2 %) and an acceptable coefficient of determination (r2 ≥0.994). These findings establish our proposed method as suitable for reliable drug quantification in clinical samples. © 2023 Elsevier B.V.