Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr

Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr Publisher Pubmed

Pourhajibagher M^{1, 2, 3} ; Chiniforush N⁴ ; Shahabi S^{4, 5} ; Sobhani S⁶ ; Monzavi MM⁶ ; Monzavi A⁴ ; Bahador A^{1, 2, 4}

Show Affiliations

1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3. Dental Implant Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
4. Laser Research Center of Dentistry (LRCD), Tehran University of Medical Sciences, Tehran, Iran
5. Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran
6. School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran

Source: Photodiagnosis and Photodynamic Therapy Published:2017

Abstract

Background Aggregatibacter actinomycetemcomitans is an important pathogen that is frequently found in various infections, particularly aggressive periodontitis. In this study, we described the outcome of the expression level of A. actinomycetemcomitans virulence factor following treatment by antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) as a photosensitizing agent. Materials and methods To determine the aPDT effect on the cell-surviving assay and expression ratio of the rcpA gene in A. actinomycetemcomitans by a colony-forming unit and relative quantitative (q) real-time PCR (qRT-PCR) assays, respectively, the proper dosing of sub-lethal aPDT was specified. Results The results of the current study showed that ICG-mediated aPDT, using 250–1000 μg/mL, showed a significant reduction in A. actinomycetemcomitans growth when compared to the control group (P < 0.05). Also, a sub-lethal dose of aPDT against A. actinomycetemcomitans was 125 μg/mL ICG, with a 30 s diode laser irradiation time at fluency of 15.6 J/cm2 that could reduce the expression of rcpA gene approximately 6-fold. Discussion aPDT with ICG could reduce the cell survival and the virulence agent of A. actinomycetemcomitans. Thus, use of the appropriate aPDT dosage can be used for the successful treatment of periodontitis in vivo. © 2017 Elsevier B.V.

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Experts (# of related papers)

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Abbas Bahador (53)

Maryam Pourhajibagher (50)

Style	Citing Format
MLA	Pourhajibagher M, et al.. "Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr." Photodiagnosis and Photodynamic Therapy, vol. 19, no. , 2017, pp. 51-55.
APA	Pourhajibagher M, Chiniforush N, Shahabi S, Sobhani S, Monzavi MM, Monzavi A, Bahador A (2017). Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr. Photodiagnosis and Photodynamic Therapy, 19(), 51-55.
Chicago	Pourhajibagher M, Chiniforush N, Shahabi S, Sobhani S, Monzavi MM, Monzavi A, Bahador A. "Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr." Photodiagnosis and Photodynamic Therapy 19, no. (2017): 51-55.
Harvard	Pourhajibagher M et al. (2017) 'Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr', Photodiagnosis and Photodynamic Therapy, 19(), pp. 51-55.
Vancouver	Pourhajibagher M, Chiniforush N, Shahabi S, Sobhani S, Monzavi MM, Monzavi A, et al.. Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr. Photodiagnosis and Photodynamic Therapy. 2017;19():51-55.
BibTex	@article{ author = {Pourhajibagher M and Chiniforush N and Shahabi S and Sobhani S and Monzavi MM and Monzavi A and Bahador A}, title = {Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr}, journal = {Photodiagnosis and Photodynamic Therapy}, volume = {19}, number = {}, pages = {51-55}, year = {2017} }
RIS	TY - JOUR AU - Pourhajibagher M AU - Chiniforush N AU - Shahabi S AU - Sobhani S AU - Monzavi MM AU - Monzavi A AU - Bahador A TI - Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr JO - Photodiagnosis and Photodynamic Therapy VL - 19 IS - SP - 51 EP - 55 PY - 2017 ER -

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