Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison

Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison Publisher

S Salarvand SAMANEH ; R Abyaneh ROMINA ; A Raee ABDORREZA ; M Yaghootikhorasani MAHDIYEH ; F Mohammadjani FARIBA ; F Nili FATEMEH ; M Aghili MAHDI ; R Ghalehtaki REZA

Source: Clinical and Translational Radiation Oncology Published:2025

Abstract

Background: Antitumor immunity, exerted by CD8+ cytotoxic T lymphocytes, plays a vital role in tumor control. Therefore, the present study was conducted to compare the amount of CD8+ tumor-infiltrating lymphocytes (TILs) before and after either long- (LCRT) or short-course radiotherapy (SCRT) in rectal cancer. Methods: This study retrospectively assessed rectal cancer patients treated by neoadjuvant radiotherapy between 2019 and 2021. Biopsy and surgical samples were subjected to immunohistochemical staining to count CD8+ TILs. The association between the post-to-pre-treatment CD8+ count ratio and treatment groups, histopathological factors, and response to treatment was assessed. Results: A total of 34 patients were included, with 23 (67.6 %) receiving LCRT and 11 (32.4 %) receiving SCRT. The mean age was 58.56 ± 13.59 years. The number and percentage of CD8+ TILs increased significantly after radiotherapy in all patients (P < 0.001). An increase in CD8+ TILs was observed in both groups, with LCRT showing a median post-to-pre-treatment count ratio of 2.77 and SCRT showing 3.1 (P = 0.127). A generalized linear multivariate model adjusting for mucinous histology, surgical grade, and pathological stages revealed that SCRT was associated with a significantly higher post-to-pre-treatment CD8+ count ratio compared to LCRT (P = 0.03). Conclusion: Our study highlights that both SCRT and LCRT significantly increase CD8+ TIL count and percentage, reflecting robust immune activation after radiotherapy in rectal cancer, with SCRT showing a higher relative increase, though not statistically significant in unadjusted analyses. After adjusting for histopathological variables, SCRT was independently associated with a greater increase in CD8+ T cells. © 2025 Elsevier B.V., All rights reserved.

Style	Citing Format
MLA	S Salarvand SAMANEH, et al.. "Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison." Clinical and Translational Radiation Oncology, vol. 54, no. , 2025, pp. -.
APA	S Salarvand SAMANEH, R Abyaneh ROMINA, A Raee ABDORREZA, M Yaghootikhorasani MAHDIYEH, F Mohammadjani FARIBA, F Nili FATEMEH, M Aghili MAHDI, R Ghalehtaki REZA (2025). Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison. Clinical and Translational Radiation Oncology, 54(), -.
Chicago	S Salarvand SAMANEH, R Abyaneh ROMINA, A Raee ABDORREZA, M Yaghootikhorasani MAHDIYEH, F Mohammadjani FARIBA, F Nili FATEMEH, M Aghili MAHDI, R Ghalehtaki REZA. "Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison." Clinical and Translational Radiation Oncology 54, no. (2025): -.
Harvard	S Salarvand SAMANEH et al. (2025) 'Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison', Clinical and Translational Radiation Oncology, 54(), pp. -.
Vancouver	S Salarvand SAMANEH, R Abyaneh ROMINA, A Raee ABDORREZA, M Yaghootikhorasani MAHDIYEH, F Mohammadjani FARIBA, F Nili FATEMEH, et al.. Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison. Clinical and Translational Radiation Oncology. 2025;54():-.
BibTex	@article{ author = {S Salarvand SAMANEH and R Abyaneh ROMINA and A Raee ABDORREZA and M Yaghootikhorasani MAHDIYEH and F Mohammadjani FARIBA and F Nili FATEMEH and M Aghili MAHDI and R Ghalehtaki REZA}, title = {Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison}, journal = {Clinical and Translational Radiation Oncology}, volume = {54}, number = {}, pages = {-}, year = {2025} }
RIS	TY - JOUR AU - S Salarvand SAMANEH AU - R Abyaneh ROMINA AU - A Raee ABDORREZA AU - M Yaghootikhorasani MAHDIYEH AU - F Mohammadjani FARIBA AU - F Nili FATEMEH AU - M Aghili MAHDI AU - R Ghalehtaki REZA TI - Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison JO - Clinical and Translational Radiation Oncology VL - 54 IS - SP - EP - PY - 2025 ER -

Style

Citing Format

MLA

S Salarvand SAMANEH, et al.. "Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison." Clinical and Translational Radiation Oncology, vol. 54, no. , 2025, pp. -.

APA

S Salarvand SAMANEH, R Abyaneh ROMINA, A Raee ABDORREZA, M Yaghootikhorasani MAHDIYEH, F Mohammadjani FARIBA, F Nili FATEMEH, M Aghili MAHDI, R Ghalehtaki REZA (2025). Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison. Clinical and Translational Radiation Oncology, 54(), -.

Chicago

S Salarvand SAMANEH, R Abyaneh ROMINA, A Raee ABDORREZA, M Yaghootikhorasani MAHDIYEH, F Mohammadjani FARIBA, F Nili FATEMEH, M Aghili MAHDI, R Ghalehtaki REZA. "Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison." Clinical and Translational Radiation Oncology 54, no. (2025): -.

Harvard

S Salarvand SAMANEH et al. (2025) 'Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison', Clinical and Translational Radiation Oncology, 54(), pp. -.

Vancouver

S Salarvand SAMANEH, R Abyaneh ROMINA, A Raee ABDORREZA, M Yaghootikhorasani MAHDIYEH, F Mohammadjani FARIBA, F Nili FATEMEH, et al.. Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison. Clinical and Translational Radiation Oncology. 2025;54():-.

BibTex

@article{ author = {S Salarvand SAMANEH and R Abyaneh ROMINA and A Raee ABDORREZA and M Yaghootikhorasani MAHDIYEH and F Mohammadjani FARIBA and F Nili FATEMEH and M Aghili MAHDI and R Ghalehtaki REZA}, title = {Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison}, journal = {Clinical and Translational Radiation Oncology}, volume = {54}, number = {}, pages = {-}, year = {2025} }

RIS

TY - JOUR
AU - S Salarvand SAMANEH
AU - R Abyaneh ROMINA
AU - A Raee ABDORREZA
AU - M Yaghootikhorasani MAHDIYEH
AU - F Mohammadjani FARIBA
AU - F Nili FATEMEH
AU - M Aghili MAHDI
AU - R Ghalehtaki REZA
TI - Cd8-Positive Lymphocyte Infiltration As a Marker of Anti-Tumor Immune Response in Rectal Cancer: Pre- and Post-Neoadjuvant Radiotherapy Comparison
JO - Clinical and Translational Radiation Oncology
VL - 54
IS -
SP -
EP -
PY - 2025
ER -

Science Communicator Platform

Authors

Abstract