Definition of an Oxidative Stress Status by Combined Assessment of Malondialdehyde and Oxidized-Ldl: A Study in Patients With Type2 Diabetes and Control Publisher



Ghazizadeh A¹ ; Khaloo P¹ ; Alemi H¹ ; Rabizadeh S¹ ; Mirmiranpour H¹ ; Esteghamati A¹ ; Nakhjavani M¹ Show Affiliations
Source: Meta Gene Published:2019

Abstract

Background: Type 2 diabetes mellitus (T2DM) is associated with an oxidative milieu that leads to increased lipid peroxidation. Main focus of this study was to understand levels and correlations of two oxidative stress indices, malondialdehyde (MDA) and oxidized low-density lipoprotein (ox-LDL) levels, in diabetes associated oxidative stress. Moreover we examined the impact of different HDL-C levels on this correlation. Material and methods: A total of 151 patients with T2DM and 45 healthy adults were enrolled in this cross-sectional study. Fasting plasma glucose, total cholesterol, triglyceride, High-Density Lipoprotein (HDL–C), low-density lipoprotein (LDL), ox-LDL and MDA were measured. Results: Linear regression analysis revealed higher levels and a positive correlation between MDA and ox-LDL in patients with T2DM (p <.001). ROC curve analysis revealed that 3.57 nmol/ml for MDA and 60.68 U/dL for ox-LDL could serve as optimum cut-off points with maximum sensitivity and specificity in predicting T2DM associated oxidative stress. Subjects with both MDA > 3.57 nmol/ml and ox-LDL > 60.68 U/dl had significantly poorer lipid profile and diabetic nephropathy indices. Patients were stratified into four quartiles based on associated HDL levels. Ox-LDL and MDA showed significant positive correlation only in HDL levels below 35 mg/dl. Conclusions: We attempted to investigate applicability of MDA and ox-LDL in predicting T2DM related oxidative stress. Present data strongly suggest that using both of MDA and ox-LDL increases their specificity in predicting oxidative milieu and diabetic nephropathy. Moreover, we showed that HDL levels above 35 mg/dl might have beneficial effect on oxidative reactions. © 2018