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The Importance of the Non-Active Site and Non-Periodical Structure Located Histidine Residue Respect to the Structure and Function of Exo-Inulinase Publisher Pubmed



Arjomand MR1, 2 ; Ahmadian G2 ; Habibirezaei M1, 3 ; Hassanzadeh M4 ; Karkhane AA2 ; Moosavimovahedi AA5, 6 ; Amanlou M4
Authors
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Authors Affiliations
  1. 1. School of Biology, College of Science, University of Tehran, Tehran, Iran
  2. 2. Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
  3. 3. Nano-Biomedicine Center of Excellence, Nanoscience and Nanotechnology Research Center, University of Tehran, Tehran, Iran
  4. 4. Department of Medicinal Chemistry & Drug Design and Development Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  6. 6. Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2017


Abstract

Here, we have studied the role of a histidine residue with the lowest solvent accessibility among other histidine residues at the end of a short connecting structure (189AELH192) of the catalytic domain of the exo-inulinase through creation of H192A mutant. Site-directed mutagenesis method was applied to create the mutant enzyme. Molecular dynamics (MD) simulations, spectroscopic, calorimetric and kinetics analysis were used to study the structural and functional consequences of His192 substitution. Accordingly, the thermo-stabilities and catalytic performance were decreased upon H192A mutation. In silico and experimental approaches evidently confirm that His192 residue of exo-inulinase possesses structural and functional importance regardless of the lack of direct interaction with the substrate or involvement in the catalytic activity of exo-inulinase. © 2017 Elsevier B.V.
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