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Effects of Heating and Acidic Solutions of Vinegar and Oxymel on Milk Coagulation for Identification and Quantification of Resulting Α-Lactalbumin and Β-Lactoglobulin Proteins in the Final Whey Product Publisher



Mirabzadeh M1 ; Khanavi M2 ; Golestani A3 ; Shamsardekani MR1 ; Rahimi R1 ; Sahraei Z4 ; Moghaddam G5 ; Khoshayand MR5 ; Hajimahmoodi M5
Authors
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Authors Affiliations
  1. 1. Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pharmacognosy, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Clinical Pharmacy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Analytical Chemistry Letters Published:2015


Abstract

The effects of vinegar and oxymel solutions (used in Iranian traditional medicine) and processing temperatures (75, 85 and 100°C) on the milk coagulation was studied to identify and determine the protein indicators of resulting whey. The HPLC (high performance liquid chromatography) analysis showed that the α-lactalbumin (ALA) and β-lactoglobulin (BLG) contents of resulting whey reduced significantly, when the coagulation temperature increased from 75 to 100°C. In both solutions and at 75°C processing temperature, the BLG of final whey protein was significantly more (∼ 60-70 %) than ALA content. The reverse process happened at 85°C, and the ALA content became much higher than BLG. A significantly higher content of ALA was recovered once the combination of oxymel and vinegar solutions with milk reached to respectively ∼ 10 and 0.3 % and milk protein was coagulated at 85°C. Overall, there is a good possibility to make a milk whey with high content of desirable ALA and negligible concentrations of BLG to convert cow's milk proteins to human's milk as it was the main intention in Iranian Traditional Medicine method. © 2015 Har Krishan Bhalla & Sons.