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An Evaluation of Photobiomodulation Effects on Human Gingival Fibroblast Cells Under Hyperglycemic Condition: An in Vitro Study Publisher Pubmed



Iranpour B1 ; Mohammadi K1 ; Hodjat M2 ; Hakimiha N3 ; Sayar F1 ; Kharazi Fard MJ2 ; Sadatmansouri S1 ; Hanna R4, 5, 6
Authors
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Authors Affiliations
  1. 1. Department of Periodontology, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  2. 2. Dental Research Centre, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Laser Application in Medical Sciences Research Centre, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Oral Surgery, King’s College Hospital, Denmark Hill, London, SE5 9RS, United Kingdom
  5. 5. Department of Restorative Dental Sciences, Faculty of Medical Sciences, UCL-Eastman Dental Institute, Rockefeller Building, London, WC1E 6DE, United Kingdom
  6. 6. Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Viale Benedetto XV, 6, Genoa, 16132, Italy

Source: Lasers in Medical Science Published:2024


Abstract

An in vitro study was designed to evaluate the effects of photobiomodulation (PBM) with 915-nm diode laser on human gingival fibroblast (HGF) cells under hyperglycemic condition. The HGF cells were cultured in Dulbecco’s modified eagle medium (DMEM) medium containing 30 mM glucose concentration for 48 h to mimic the hyperglycemic condition. Subsequently, the cells received three sessions of PBM (915 nm, continuous emission mode, 200 mW, energy density values of 3.2, 6, and 9.2 J/cm2). Twenty-four hours post-irradiation, cell proliferation, expression of interleukin 6 (IL-6), and vascular endothelial growth factor (VEGF) were assessed with MTT and real-time polymerase chain reaction (PCR) tests, respectively. Also, reactive oxygen species (ROS) production was measured using CM-H2DCFDA fluorimetry. No changes were detected in the cell proliferation rate between the high glucose control group and laser-treated cells, while VEGF and IL-6 gene expression levels increased significantly after PBM in the high glucose-treated cells group. ROS level was significantly decreased in the irradiated cells in high-glucose medium compared with the high glucose control group. Our study revealed the inductive role of 915-nm–mediated PBM on VEGF and the inflammatory response while concurrently reducing reactive oxygen species production in HGF cells in hyperglycemic conditions. © 2023, The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature.