Subtyping Β-Lactamase-Producing Escherichia Coli Strains Isolated From Patients With Uti by Mlva and Pfge Methods Publisher



Dehkharghani AD¹ ; Haghighat S¹ ; Farzami MR² ; Douraghi M³ ; Rahbar M² Show Affiliations
Source: Iranian Journal of Basic Medical Sciences Published:2021

Abstract

Objective(s): Strain subtyping is an important epidemiological tool to trace contamination, determine clonal relationships between different strains, and the cause of outbreaks. Current subtyping methods, however, yield less than optimal subtype discrimination. Pulsed-field gel electrophoresis is the gold standard method for Escherichia coli and Multiple-Locus Variable-number tandem repeat Analysis (MLVA) is a rapid PCR-based method. The purpose of this study was to evaluate MLVA and pulsed-field gel electrophoresis (PFGE) methods for subtyping β -lactamase-producing E. coli strains isolated from urinary tract infections. Materials and Methods: Overall, 230 E. coli isolates from patients with urinary tract infections were examined for antimicrobial susceptibility testing. 10-loci and 7-loci MLVA and PFGE methods were used for molecular typing of β -lactamase-producing E. coli isolates. Results: Out of 230 isolates, 130 (56.5%) β -lactamase-producing E. coli isolates were found in this study. The diversity indices of the VNTR loci showed an average diversity of 0.48 and 0.54 for 7-loci and 10-loci MLVA, respectively. The discriminatory power of PFGE showed a value of 0.87. The discordance between the methods was high. Conclusion: Our study showed that PFGE is more discriminatory than MVLA. MLVA is a PCR- based method and can generate unmistakable data, in contrast to PFGE. Optimization of polymorphic VNTR is essential to improve the discriminatory power of MLVA based on geographical region. © 2021 Mashhad University of Medical Sciences. All rights reserved.