The Evaluation of the Ability of Carotenoids Extracted From Shrimp Waste to Prevent Lipid Oxidation in Crude Oils; [رد نویسادیسکادیپیل زا تعنامم رد وگیم تاعیاض زا یجارختسا یاهدیئونتوراک تیلباق یسررب و جارختسا ماخ یاه نغور]



Ghanati K ; Aghamohammadi A ; Akbari N ; Mirzaei G ; Karimisarzameleh M ; Dehghani MH ; Sadighara P
Source: Iranian Journal of Health and Environment Published:2025

Abstract

Background and Objective: A large amount of waste is generated from shrimp farming, primarily consisting of shrimp heads and shells. These wastes are often discharged into aquatic environments, where they act as pollutants and threaten the survival of other aquatic organisms. Therefore, reusing shrimp waste can help reduce water pollution and mitigate its environmental impact. Material and Methods: In this study, carotenoids from shrimp waste were extracted using three methods: acidic, enzymatic, and alkaline. The amount of carotenoids was quantified for each extraction method. Their antioxidant effects on crude sunflower oil, in the presence of copper sulfate, were then evaluated using the TBARS assay. In this method, malondialdehyde levels are measured as an indicator of lipid oxidation. For this purpose, equal amounts of the extracted carotenoids were added to crude sunflower oil along with copper sulfate and placed on a shaker for 24 hours. Subsequently, the malondialdehyde content was measured. Results: The highest carotenoid yield was obtained using the alkaline extraction method. In the TBARS assay, the alkaline method also demonstrated the greatest inhibition of lipid peroxidation in the presence of copper sulfate. Conclusion: It can be concluded that the alkaline method is a simple and cost-effective approach for extracting carotenoids from shrimp waste. These carotenoids are bioactive and valuable compounds with potential applications in the pharmaceutical and food industries. Copyright © 2025 Iranian Association of Environmental Health and Tehran University of Medical Sciences. Published by Tehran University of Medical Sciences. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (https://creativecommons.org/licenses/by-nc/4.0/). Noncommercial uses of the work are permitted provided the original work is properly cited.