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Sustain Release of Loaded Insulin Within Biomimetic Hydrogel Microsphere for Sciatic Tissue Engineering in Vivo Publisher Pubmed



Zolfagharzadeh V1 ; Ai J2 ; Soltani H1 ; Hassanzadeh S3, 4 ; Khanmohammadi M4
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Authors Affiliations
  1. 1. Chemical Engineering Department, Engineering Faculty, Islamic Azad University, Ahar Branch, Ahar, Iran
  2. 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Eye Research Center, Five Senses Health Research Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Skull based Research Center, The Five Senses Institute, Hazrat Rasoul Akram Hospital, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2023


Abstract

We developed insulin loaded biomimetic microsphere by laccase-mediated crosslinking using a microfluidic device in the water-in-oil emulsion system as an injectable vehicle for the repair of sciatic tissue. Aqueous polymeric solution of phenol-substituted hyaluronic acid (HAPh) and collagen (ColPh) containing insulin and laccase flowed from the inner channel into oil flow within an outer channel which leads formation of hydrogel microsphere. The physical properties of prepared specimens including swelling rate, mechanical resistance and the prolonged release rate of microspheres proved applicability of fabricated vehicles for tissue engineering and drug delivery systems. The growth profile and behavior of cells in microspheres indicated cytocompatibility of the method and prepared vehicles for microtissue development. Histopathological examination revealed a significant increase in axonal regeneration, and remyelination process in injured sciatic nerve following treatment with HAPh/ColPh microspheres containing insulin compared to control groups. Also, the functional characteristic of sciatic tissue showed that the presence of biomimetic microsphere and insulin simultaneously had improved sciatic tissue functions including functional sciatic index (SFI) values, reaction to hot plate and muscle weight of rats. In summary, the results proved that composite biomimetic microspheres containing insulin effectively improved nerve regeneration in the rat model. © 2022
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