A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus

A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus Publisher

Khodabakhsh T¹ ; Arabi A¹ ; Pakzad P¹ ; Gheflat S² ; Bahreinipour A³ ; Bandehpour M^{2, 4}

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1. Department of Microbiology, School of Biology Sciences, North Branch, Islamic Azad University, Tehran, Iran
2. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Iran Veterinary Organization (IVO), Tehran, Iran
4. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Korean Journal of Microbiology and Biotechnology Published:2019

Abstract

Brucellosis is one of the most important zoonotic diseases that lead to a great amount of economic losses. Prevention and diagnosis are both necessary to eradicate this disease. The identification and evaluation of different antigens of Brucella spp. play a key role in the progress of diagnostic programs. In this study, we designed, produced, and evaluated a 24-kDa polypeptide containing antigenic epitopes of VirB2, 3, and 9 of Brucella abortus for use with the ELISA kit. The produced polypeptide is appropriate for diagnosing brucellosis in bovines by a laboratory diagnostic kit, with 100% sensitivity and 97.5% specificity. © 2019 The Korean Society for Microbiology and Biotechnology.

Experts (# of related papers)

Mohammad Reza Khorramizadeh (1)

Style	Citing Format
MLA	Khodabakhsh T, et al.. "A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus." Korean Journal of Microbiology and Biotechnology, vol. 47, no. 1, 2019, pp. 158-163.
APA	Khodabakhsh T, Arabi A, Pakzad P, Gheflat S, Bahreinipour A, Bandehpour M (2019). A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus. Korean Journal of Microbiology and Biotechnology, 47(1), 158-163.
Chicago	Khodabakhsh T, Arabi A, Pakzad P, Gheflat S, Bahreinipour A, Bandehpour M. "A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus." Korean Journal of Microbiology and Biotechnology 47, no. 1 (2019): 158-163.
Harvard	Khodabakhsh T et al. (2019) 'A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus', Korean Journal of Microbiology and Biotechnology, 47(1), pp. 158-163.
Vancouver	Khodabakhsh T, Arabi A, Pakzad P, Gheflat S, Bahreinipour A, Bandehpour M. A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus. Korean Journal of Microbiology and Biotechnology. 2019;47(1):158-163.
BibTex	@article{ author = {Khodabakhsh T and Arabi A and Pakzad P and Gheflat S and Bahreinipour A and Bandehpour M}, title = {A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus}, journal = {Korean Journal of Microbiology and Biotechnology}, volume = {47}, number = {1}, pages = {158-163}, year = {2019} }
RIS	TY - JOUR AU - Khodabakhsh T AU - Arabi A AU - Pakzad P AU - Gheflat S AU - Bahreinipour A AU - Bandehpour M TI - A New Elisa Kit Based on Antigenic Epitopes for Diagnosing Brucella Abortus JO - Korean Journal of Microbiology and Biotechnology VL - 47 IS - 1 SP - 158 EP - 163 PY - 2019 ER -

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