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A Novel Method for the Simultaneous Determination of 5-Fluorouracil and Oxaliplatin in New Biodegradable Phbv/Plga Nanoparticles Publisher



Handali S1 ; Ramezani Z1 ; Moghimipour E1, 2 ; Rezaei M3 ; Dorkoosh FA4, 5
Authors
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Authors Affiliations
  1. 1. Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  2. 2. Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
  3. 3. Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  4. 4. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Medical Biomaterial Research Centre (MBRC), Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of the Iranian Chemical Society Published:2019


Abstract

The aim of the present study was to develop a novel HPLC method for the simultaneous determination of 5-fluorouracil (5-FU) and oxaliplatin (OXA) in the PHBV/PLGA nanoparticles (NPs). Chromatographic separation was performed using C18 column at a detection wavelength of 260 nm. The mobile phase consisted of 0.02 M phosphate buffer (pH 4) and methanol (90:10, V/V) and flow rate was 0.8 mL min −1 . Column temperature was also maintained at 30 °C. The calibration curves displayed linear concentration ranges of 0.05–40 µg mL −1 for 5-FU and 0.5–80 µg mL −1 for OXA with correlation coefficients of 0.998. The LOD and LOQ were, respectively, 0.01 and 0.03 µg mL −1 for 5-FU and 0.13 and 0.44 µg mL −1 for OXA. The values of intra- and inter-day precision and accuracy were within acceptable limits. According to the results, the method was found to be simple, reliable, rapid, and reproducible. In addition, the HPLC assay method presented was successfully applied to the simultaneous determination of 5-FU and OXA in PHBV/PLGA NPs. © 2018, Iranian Chemical Society.