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Role of Stromal Derived Factor-1A (Sdf-1A) for Spermatogenesis of Busulfan-Injured Rats Publisher Pubmed



Khanlarkhani N1 ; Mortezaee K2 ; Amidi F1 ; Kharazinejad E3 ; Beyer C4 ; Baazm M5 ; Pasbakhsh P1 ; Pazhohan A1 ; Sobhani A1 ; Zendedel A4
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
  3. 3. Department of Anatomy, School of Medicine, Abadan University of Medical Sciences, Abadan, Iran
  4. 4. Institute of Neuroanatomy, RWTH Aachen University, Aachen, Germany
  5. 5. Department of Anatomical Sciences, Arak University of Medical Sciences, Arak, Iran

Source: Reproductive Toxicology Published:2017


Abstract

SDF-1a is a member of CXC chemokine family that plays a crucial role in stem cell migration, cell apoptosis and development. The role of intra-scrotal administration of SDF-1a in spermatogenesis of busulfan-treated rats was investigated in this study. Two injections of busulfan (15 mg/kg) with a 14 days interval between were given intraperitoneally to male Wistar rats. Rats were then treated for seven days with 500 ng/mL SDF-1a. Real-time PCR and immunohistochemistry were performed for evaluation of various cell markers for proliferation and spermatogenesis, and sperm parameters were assessed. In the SDF-1a group, there was a significant increase in testis weight, sperm count and viability. DAZL, DDX4, and TP2 showed increased expression levels in the SDF-1a group. PCNA and BrdU revealed highest expression rates in the SDF-1a group (p ≤ 0.0001). These findings showed the protective role of SDF-1a in busulfan-induced testis injury most likely through stimulation of SSCs proliferation. © 2017 Elsevier Inc.