Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Transient Expression of Biologically Active Anti-Rabies Virus Monoclonal Antibody in Tobacco Leaves Publisher



Shafaghi M1 ; Maktoobian S1 ; Rasouli R2 ; Howaizi N3 ; Ofoghi H4 ; Ehsani P1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
  2. 2. Department of Medical Nanotechnology, School of Medicine International Campus, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. WHO Collaborating Centre for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

Source: Iranian Journal of Biotechnology Published:2018


Abstract

Background: Rabies virus is a neurotropic virus that causes fatal, but, a preventable disease in mammals. Administration of rabies immunoglobulin (RIG) is essential for the post-exposure of the prophylaxis to prevent the disease. However, replacement of polyclonal RIGs with alternative monoclonal antibodies (MAbs) that are capable of neutralizing rabies virus has been recommended. Objectives: Here, we have investigated the transient expression of the full-size human MAb against rabies virus glycoprotein; the MAb SO57 in the tobacco plants using vacuum agro-infiltration. Previously, stably transformed plants expressing the MAb have been reported. Materials and Methods: In this study three vectors carrying the codon-optimized genes for the heavy or light chain and p19 silencing-suppressor were constructed. These vectors were co-infiltrated into Nicotiana tabacum leaves and the transgenes were expressed. Results: Dot blot, Western blotting, ELISA, and in vitro neutralization assays of the plant extracts showed that the human MAb could assemble in tobacco leaves and was able to neutralize rabies virus. Conclusions: This study is the first report of transient expression of human MAb SO57 gene in tobacco plant within a few days after vacuum agro-infiltration. © 2017 The Author(s).
Related Docs
View other Related Docs
1. Plant-Based Vaccines and Cancer Therapy: Where Are We Now and Where Are We Going?, Pharmacological Research (2021)
2. Deferoxamine Preconditioning of Neural-Like Cells Derived From Human Wharton’S Jelly Mesenchymal Stem Cells As a Strategy to Promote Their Tolerance and Therapeutic Potential: An in Vitro Study, Cellular and Molecular Neurobiology (2016)
3. Thermoalkalophilic Lipase From an Extremely Halophilic Bacterial Strain Bacillus Atrophaeus Fshm2: Purification, Biochemical Characterization and Application, Biocatalysis and Biotransformation (2017)
4. Antioxidative Responses of Nostoc Ellipsosporum and Nostoc Piscinale to Salt Stress, Journal of Applied Phycology (2019)
5. Studying Neuroprotective Effect of Atorvastatin As a Small Molecule Drug on High Glucose-Induced Neurotoxicity in Undifferentiated Pc12 Cells: Role of Nadph Oxidase, Metabolic Brain Disease (2017)
6. Differential Pulse Voltammetric Immunosensor for Direct and Label-Free Detection of Vegf Using Variable Domain of Heavy-Chain Antibody Displaying Phage, Microchemical Journal (2024)
7. Surface Plasmon Resonance Based Biosensor for Discovery of New Matrix Metalloproteinase-9 Inhibitors, Sensors and Actuators# B: Chemical (2018)
8. Phytocatalytic and Cytotoxic Activity of the Purified Laccase From Bled Resin of Pistacia Atlantica Desf., International Journal of Biological Macromolecules (2021)
Experts (# of related papers)
View all Related Experts
Mohammad Ali Faramarzi (5)
Zahra Pourpak (2)
Other Related Docs
9. Immunoproteomics of Tree of Heaven (Ailanthus Atltissima) Pollen Allergens, Journal of Proteomics (2017)
10. Concomitant Abuse of Methadone and Methamphetamine Could Impair Spatial Learning and Memory in Male Rats, Learning and Motivation (2019)
11. Assessment of Recombinant A2-Latex Agglutination Test (Ra2-Lat) and Ra2-Elisa for Detection of Canine Visceral Leishmaniasis: A Comparative Field Study With Direct Agglutination Test in Northwestern Iran, Iranian Journal of Parasitology (2018)
12. Enhancing Activity and Thermostability of Lipase a From Serratia Marcescens by Site-Directed Mutagenesis, Enzyme and Microbial Technology (2016)
13. Rational Design Toward Developing a More Efficient Laccase: Catalytic Efficiency and Selectivity, International Journal of Biological Macromolecules (2018)
14. An Organic Solvent-Tolerant Lipase of Streptomyces Pratensis Mv1 With the Potential Application for Enzymatic Improvement of N6/N3 Ratio in Polyunsaturated Fatty Acids From Fenugreek Seed Oil, Journal of Food Science and Technology (2021)
15. Correlation Between Polyphenol Oxidase (Ppo) Activity and Total Phenolic Contents in Crocus Sativus L. Corms During Dormancy and Sprouting Stages, Pharmacognosy Magazine (2017)
16. Molecular Design, Expression and Evaluation of Pasylated Human Recombinant Erythropoietin With Enhanced Functional Properties, Protein Journal (2017)
17. Anther Development and Microsporogenesis in Date Palm (Phoenix Dactylifera L.), Pakistan Journal of Botany (2017)
18. Evaluation and Phylogenetic Analysis of Regular Rabies Virus Vaccine Strains, Archives of Iranian Medicine (2018)
19. Incorporation of Nanoalumina Improves Mechanical Properties and Osteogenesis of Hydroxyapatite Bioceramics, ACS Biomaterials Science and Engineering (2018)
20. Assessment of the Inhibitory Effects of Ficin-Hydrolyzed Gelatin Derived From Squid (Uroteuthis Duvauceli) on Breast Cancer Cell Lines and Animal Model, Iranian Journal of Allergy# Asthma and Immunology (2018)