Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196

Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196 Publisher Pubmed

Javid M¹ ; Nickavar B¹ ; Vahidi H¹ ; Faramarzi MA²

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1. Department of Pharmaceutical Biotechnology and Pharmacognosy, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, P.O. Box 14155-6153, Tehran, Iran
2. Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran, 1417614411, Iran

Source: Steroids Published:2018

Abstract

Microbial transformations are capable of producing steroid substances difficult to synthesize by chemical methods. Strains belonging to the genus Aspergillus are effective facilitators of microbial biotransformations due to their enzymatic diversity. In this study, the biotransformation of progesterone by the fungus Aspergillus sojae (A. sojae) PTCC 5196 was examined. Analysis of the bioconversion process revealed that progesterone was converted to testololactone through a three-step pathway (17β-acetyl side chain cleavage, 17β-hydroxyl oxidation, and oxygenative lactonization of 17-ketone), indicating the presence of Baeyer-Villiger monooxygenase (BVMO) activity in the fungal strain. GC analysis confirmed the production of testololactone with a yield of 99% in 24 h. Faster testololactone production was induced in the presence of both C-21 (progesterone) and C-19 (androstenedione, testosterone, and dehydroepiandrosterone [DHEA]) steroid substances. Due to the high biotransformation rate observed in the present study, A. sojae may be a novel and promising candidate in the production of testololactone. © 2018

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Mohammad Ali Faramarzi (1)

Style	Citing Format
MLA	Javid M, et al.. "Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196." Steroids, vol. 140, no. , 2018, pp. 52-57.
APA	Javid M, Nickavar B, Vahidi H, Faramarzi MA (2018). Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196. Steroids, 140(), 52-57.
Chicago	Javid M, Nickavar B, Vahidi H, Faramarzi MA. "Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196." Steroids 140, no. (2018): 52-57.
Harvard	Javid M et al. (2018) 'Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196', Steroids, 140(), pp. 52-57.
Vancouver	Javid M, Nickavar B, Vahidi H, Faramarzi MA. Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196. Steroids. 2018;140():52-57.
BibTex	@article{ author = {Javid M and Nickavar B and Vahidi H and Faramarzi MA}, title = {Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196}, journal = {Steroids}, volume = {140}, number = {}, pages = {52-57}, year = {2018} }
RIS	TY - JOUR AU - Javid M AU - Nickavar B AU - Vahidi H AU - Faramarzi MA TI - Baeyer-Villiger Oxidation of Progesterone by Aspergillus Sojae Ptcc 5196 JO - Steroids VL - 140 IS - SP - 52 EP - 57 PY - 2018 ER -

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