Electrospun Hyaluronic Acid–Poly Vinylalcohol Mat Loaded With Micrococcus Luteus: A Biocompatible Antibacterial Scaffold for Acute Wound Healing Publisher



Maboudi A ; Forouzideh N ; Norouzi P ; Haghi F ; Hashemikia S ; Soltanoghli M ; Aslani MR
Source: Chemical Engineering Journal Published:2025

Abstract

Infectious wounds pose significant challenges, especially with the growing issue of antibiotic resistance, which makes traditional antibiotic treatment less effective. For this reason, recent research has been seeking alternative types of treatment using probiotics. This research evaluate to was to fabricate and assess electrospun hyaluronic acid/polyvinyl alcohol (PVA) fibers incorporating Micrococcus luteus for wound management. The PVA/hyaluronic acid fibers were fabricated with electrospinning and crosslinked by dry heat treatment. Fiber morphology before and after crosslinking was examined using scanning electron microscopy (SEM). Further tests, such as Fourier-transform infrared spectroscopy (FTIR) and mechanical strength analysis, were conducted on the optimized crosslinked sample. Water vapor transmission rate (WVTR) was also measured to evaluate suitability for wound dressing applications. Micrococcus luteus in polyethylene oxide (PEO) was electrosprayed on the fibrous mat, and probiotic viability was tracked for 28 days. Antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa were evaluated. Biocompatibility and wound healing potential were evaluated through MTT and scratch closure tests at 0, 4, and 24 h. The fibers produced were 202 ± 23 nm, with a decrease in diameter following crosslinking. FTIR verified the esterification of the polymers. Mechanical testing indicated appropriate ability for wound application, while WVTR results demonstrated the material's capability to maintain a moist environment. The probiotic remained viable and effectively inhibited both pathogens. MTT results showed over 80 % cell viability, demonstrating high biocompatibility. The scratch assay also demonstrated full wound closure within 24 h, confirming the wound dressing's strong healing potential. © 2025 Elsevier B.V., All rights reserved.