Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach

Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach Publisher Pubmed

Saberi R^{1, 2, 3} ; Fakhar M^{1, 4} ; Hajjaran H³ ; Ataeipirkooh A⁵ ; Mohebali M³ ; Taghipour N⁶ ; Ziaei Hezarjaribi H⁴ ; Moghadam Y¹ ; Bagheri A⁴

Show Affiliations

1. Pediatric Infectious Diseases Research Center, Communicable Diseases Institute, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2. Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
3. Center for Research of Endemic Parasites of Iran, Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4. Toxoplasmosis Research Center, Communicable Diseases Institute, Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
5. Department of Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
6. Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: International Journal of Infectious Diseases Published:2020

Abstract

Objective: Leishmania RNA virus (LRV) is a double-stranded RNA (dsRNA) virus that circulates within many species of the Leishmania parasite. In this study, we aimed to investigate the presence of LRV2 circulating in Leishmania isolates in an old focus of ZCL located in northeastern of Iran. Methods: Leishmania isolates were collected from 85 patients that confirmed to have cutaneous leishmaniasis (CL) based on parasitological examination. To identify the Leishmania isolates, species-specific primer sets were applied for molecular identification. The presence of LRV2 was performed by RdRp-semi nested-PCR. The genetic diversity were calculated using MEGA and DnaSP. To assess haplotype diversity, 31 LRV2 strains in different regions were surveyed using analysis a 292-bp section of the RdRp sequences. Results: Out of 85 patients, 83 (97.6 %) were diagnosed with L. major and 2 (2.4 %) with L. tropica. LRV2 virus was detected in 59 (69.4%) of the CL cases. For the first time, LRV2 was reported in one L. tropica strain in Iran. The current LRV2 sequences indicated the highest similarities to an Old World LRV2. Moreover, 10 unique haplotypes were identified based on the analyzed sequences of the RdRp gene. Conclusions: Our results indicated the highest occurrence of Leishmania/LRV2 co-circulation in this known ZCL focus from northeastern Iran. Phylogenetic analyses of LRV2 sequences confirmed that these isolates belong to the order of LRV2 from the Old World. This study offered an insight into LRV2 haplotype that the informative issue can be used for genetic research of LRV2 in other regions. © 2020 The Author(s)

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Mahdi Mohebali (29)

Homa Hajjaran (13)

Style	Citing Format
MLA	Saberi R, et al.. "Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach." International Journal of Infectious Diseases, vol. 101, no. , 2020, pp. 6-13.
APA	Saberi R, Fakhar M, Hajjaran H, Ataeipirkooh A, Mohebali M, Taghipour N, Ziaei Hezarjaribi H, Moghadam Y, Bagheri A (2020). Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach. International Journal of Infectious Diseases, 101(), 6-13.
Chicago	Saberi R, Fakhar M, Hajjaran H, Ataeipirkooh A, Mohebali M, Taghipour N, Ziaei Hezarjaribi H, Moghadam Y, Bagheri A. "Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach." International Journal of Infectious Diseases 101, no. (2020): 6-13.
Harvard	Saberi R et al. (2020) 'Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach', International Journal of Infectious Diseases, 101(), pp. 6-13.
Vancouver	Saberi R, Fakhar M, Hajjaran H, Ataeipirkooh A, Mohebali M, Taghipour N, et al.. Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach. International Journal of Infectious Diseases. 2020;101():6-13.
BibTex	@article{ author = {Saberi R and Fakhar M and Hajjaran H and Ataeipirkooh A and Mohebali M and Taghipour N and Ziaei Hezarjaribi H and Moghadam Y and Bagheri A}, title = {Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach}, journal = {International Journal of Infectious Diseases}, volume = {101}, number = {}, pages = {6-13}, year = {2020} }
RIS	TY - JOUR AU - Saberi R AU - Fakhar M AU - Hajjaran H AU - Ataeipirkooh A AU - Mohebali M AU - Taghipour N AU - Ziaei Hezarjaribi H AU - Moghadam Y AU - Bagheri A TI - Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach JO - International Journal of Infectious Diseases VL - 101 IS - SP - 6 EP - 13 PY - 2020 ER -

Style

Citing Format

MLA

Saberi R, et al.. "Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach." International Journal of Infectious Diseases, vol. 101, no. , 2020, pp. 6-13.

APA

Saberi R, Fakhar M, Hajjaran H, Ataeipirkooh A, Mohebali M, Taghipour N, Ziaei Hezarjaribi H, Moghadam Y, Bagheri A (2020). Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach. International Journal of Infectious Diseases, 101(), 6-13.

Chicago

Saberi R, Fakhar M, Hajjaran H, Ataeipirkooh A, Mohebali M, Taghipour N, Ziaei Hezarjaribi H, Moghadam Y, Bagheri A. "Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach." International Journal of Infectious Diseases 101, no. (2020): 6-13.

Harvard

Saberi R et al. (2020) 'Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach', International Journal of Infectious Diseases, 101(), pp. 6-13.

Vancouver

Saberi R, Fakhar M, Hajjaran H, Ataeipirkooh A, Mohebali M, Taghipour N, et al.. Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach. International Journal of Infectious Diseases. 2020;101():6-13.

BibTex

@article{ author = {Saberi R and Fakhar M and Hajjaran H and Ataeipirkooh A and Mohebali M and Taghipour N and Ziaei Hezarjaribi H and Moghadam Y and Bagheri A}, title = {Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach}, journal = {International Journal of Infectious Diseases}, volume = {101}, number = {}, pages = {6-13}, year = {2020} }

RIS

TY - JOUR
AU - Saberi R
AU - Fakhar M
AU - Hajjaran H
AU - Ataeipirkooh A
AU - Mohebali M
AU - Taghipour N
AU - Ziaei Hezarjaribi H
AU - Moghadam Y
AU - Bagheri A
TI - Presence and Diversity of Leishmania Rna Virus in an Old Zoonotic Cutaneous Leishmaniasis Focus, Northeastern Iran: Haplotype and Phylogenetic Based Approach
JO - International Journal of Infectious Diseases
VL - 101
IS -
SP - 6
EP - 13
PY - 2020
ER -

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