Biosynthesis of Senps by Mycobacterium Bovis and Their Enhancing Effect on the Immune Response Against Hbs Antigens: An in Vivo Study Publisher



Mavandadnejad F¹ ; Yazdi MH^{1, 2} ; Hassanzadeh SM³ ; Mahdavi M² ; Faramarzi MA¹ ; Pazokitoroudi H⁴ ; Shahverdi AR^{1, 2} Show Affiliations
Source: IET Nanobiotechnology Published:2018

Abstract

Conventional hepatitis B vaccine contains alum but is less effective to induce Th1 response. Selenium nanoparticles and Bacillus Calmette-Guerin were reported as immune modulators. In this study, SeNPs were extracted from Mycobacterium bovis and characterised. SeNPs were mixed with HBs-Ag and administered to the mice to investigate he immune response pattern. With an addition of Se ions at a sub-inhibitory concentration to the Sauton medium broth after 24 h, SeNPs were extracted from M. bovis and characterised by Fourier transform infrared spectroscopy, dynamic light scattering, atomic forcemicroscopy, energy dispersive X-ray spectrum, transmission electron microscopy, and thermogravimetric analysis. Furthermore, female inbred BALB/c mice were injected subcutaneously on the first, 14th, 28th day with 100 and 200 μg doses of that SeNPs supplemented with HBs-Ag vaccine. Later, the total antibody, isotypes of Immunoglobulin G1, Interlukin 4, and interferon-γ were measured by enzye-linked immunosorbent assay. The size of the SeNPs was <150 nm. Level of total antibody and immunoglobulin G2a increased significantly in the group that received 200 μg/ml nano selenium extracted from M. bovis. SeNPs in dose of 200 μg coated with organic materials of M. bovis could induce an influential immune response in relation to the conventional HBs-Ag vaccine. © The Institution of Engineering and Technology 2017.