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Enhancement of Resistance to Chemo-Radiation by Hsa-Mir-1290 Expression in Glioblastoma Cells Publisher Pubmed



Khalighfard S1, 2 ; Kalhori MR3 ; Haddad P1 ; Khori V4 ; Alizadeh AM2, 5
Authors
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Authors Affiliations
  1. 1. Radiation Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Breast Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
  4. 4. Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  5. 5. Cancer Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: European Journal of Pharmacology Published:2020


Abstract

One of the resistance mechanisms to chemo-radiation is the efficiency of the DNA repair systems. MicroRNAs can alter the expression of their involved proteins; therefore, it may lead to a change in the response of cancer cells to adjuvant treatments. Here, the present study is aimed to investigate the role of hsa-miR-1290 on the chemo-radiation resistance and the target genes in the glioblastoma cells. First, we altered miR-1290 expression in the U-87 cells by using hsa-miR-1290 mimic and anti-miR-1290. Then, the Annexin V, CCK-8, MTT, colony formation, invasion, migration, and wound healing tests were utilized to study hsa-miR-1290 influences on cellular behavior such as proliferation, apoptosis, and metastasis. Moreover, the qRT-PCR and Western blot analyses were used to evaluate the effects of miR-1290 on the SOCS4 gene expression. Our results represented that the overexpression of miR-1290 could increase cell proliferation, migration, invasion, and resistance to chemo-radiation. The results showed miR-1290 directly targeted the 3՛UTR of the SOCS4 gene and suppressed its expression. Moreover, the suppression of hsa-miR-1290 led to an increase of apoptosis and cellular sensitivity to chemotherapy drugs and could also lead to decrease cell proliferation, migration, and invasion. Our findings proposed that miR-1290 can function as a novel oncomiR in glioblastoma cells by regulating its downstream genes such as SOCS4. Moreover, hsa-miR-1290 may be employed as a therapeutic target for clinical therapy of glioblastoma. © 2020 Elsevier B.V.