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Notch Signaling Pathway in Cumulus Cells Reflecting Zygote and Embryo Quality in Polycystic Ovary Syndrome Publisher Pubmed



Masoudi M1, 2, 3 ; Yamini N4 ; Salehi F5 ; Aflatoonian R6 ; Kutenaee MA7 ; Esfandiyari S8 ; Hosseini E9 ; Asadi E8 ; Najarkolaei AH10 ; Mortezaee K11 ; Moini A6, 12, 13 ; Abolhassani F8 ; Salehi E7
Authors
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Authors Affiliations
  1. 1. Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  2. 2. Department of Laboratory Sciences, Faculty of Para-Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  3. 3. Fatemiyeh Shiraz Institute of Higher Education, Shiraz, Iran
  4. 4. Department of ART, Embryology Laboratory, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Agricultural Biotechnology, Shahed University, Tehran, Iran
  6. 6. Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
  7. 7. Fertility and Infertility Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  8. 8. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Department of Gynecology and Obstetrics, School of Medicine, the IVF Clinic, Mousavi Hospital, Zanjan University of Medical Sciences, Zanjan, Iran
  10. 10. Department of Gynecology and Obstetrics, Mahdiyeh Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  11. 11. Cancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
  12. 12. Department of Gynecology and Obstetrics, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran
  13. 13. Breast Disease Research Center(BDRC), Tehran University of Medical Sciences, Tehran, Iran

Source: Archives of Gynecology and Obstetrics Published:2021


Abstract

Purpose: The present study aimed to explore the associations between the expression pattern of molecules in the Notch pathway in the cumulus cells of polycystic ovary syndrome (PCOS) patients and the quality of zygotes and embryos. Methods: A total of 200 cumulus complexes surrounding mature oocytes were obtained from 40 patients with and without PCOS undergoing intracytoplasmic sperm injection (ICSI). The expressions of Notch-1, Notch-2, and Notch-3 genes were examined by Reverse Transcription Q-PCR assay. Moreover, immunocytochemistry was performed for the expressions of Jagged-1 and Jagged-2 proteins. The correlations between the Notch receptors and their ligand expressions and the qualities of the zygote and embryo were investigated. Results: The expression levels of Notch-2, Notch-3, Jagged-1, and Jagged-2 were significantly lower in patients with PCOS than in normal women (p < 0.05), while Notch-1 showed no meaningful difference between the groups. A positive correlation was found between Notch-1 and embryo quality. Furthermore, only Notch-2 and Jagged-2 marginally correlated with zygote quality. Conclusion: The data of the present study indicated that evaluating the molecules in the Notch pathway in PCOS patients’ cumulus cells provides a novel approach to predict the zygote and embryo quality. However, further studies on a larger population are needed to validate this finding. © 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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