Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Genotyping of Clinical and Environmental Aspergillus Flavus Isolates From Iran Using Microsatellites Publisher Pubmed



Khodavaisy S1, 2 ; Badali H3 ; Rezaie S1 ; Nabili M3 ; Moghadam KG4 ; Afhami S5 ; Hagen F6 ; Aala F2 ; Hashemi SJ1 ; Meis JF6, 7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Mycology and Parasitology, Tehran University of Medical Science, Tehran, Iran
  2. 2. Department of Medical Mycology and Parasitology, Kurdistan University of Medical Science, Sanandaj, Iran
  3. 3. Department of Medical Mycology and Parasitology, Mazandaran University of Medical Science, Sari, Iran
  4. 4. Department of Pulmonary Diseases, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Infectious Diseases, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, Netherlands
  7. 7. Department of Medical Microbiology, Radboudumc, Nijmegen, Netherlands

Source: Mycoses Published:2016


Abstract

Aspergillus flavus is the second most important Aspergillus species causing human infections in tropical countries. Despite an increasing number of infections of A. flavus in Iran, the molecular epidemiology of clinical and environmental strains has not been well studied. We used a panel of nine microsatellite markers to analyse the genetic relatedness of A. flavus. Microsatellite typing of 143 (n = 119 clinical and n = 24 environmental) isolates demonstrated 118 different genotypes. A possible outbreak at a pulmonary ward was discovered. The discriminatory power for the individual markers ranged from 0.4812 to 0.9457 and the panel of all nine markers combined yielded a diversity index of 0.9948. This high-resolution typing method assists in better understanding of the molecular epidemiology of A. flavus. © 2016 Blackwell Verlag GmbH.
Related Docs
1. A Review on Molecular Typing Methods for Aspergillus Species, Journal of Mazandaran University of Medical Sciences (2015)
2. Genetic Diversity and in Vitro Antifungal Susceptibility of 200 Clinical and Environmental Aspergillus Flavus Isolates, Antimicrobial Agents and Chemotherapy (2017)
3. Molecular Typing of Clinical and Environmental Aspergillus Fumigatus Isolates From Iran Using Microsatellites, Current Medical Mycology (2021)
4. Voriconazole Resistance Genes in Aspergillus Flavus Clinical Isolates, Journal de Mycologie Medicale (2020)
5. In Vitro Activities of Five Antifungal Agents Against 199 Clinical and Environmental Isolates of Aspergillus Flavus, an Opportunistic Fungal Pathogen, Journal de Mycologie Medicale (2016)
6. Clinical Epidemiology of Pulmonary Aspergillosis in Hospitalized Patients and Contribution of Cyp51a, Yap1, and Cdr1b Mutations to Voriconazole Resistance in Etiologic Aspergillus Species, European Journal of Clinical Microbiology and Infectious Diseases (2023)
7. The Utilization of Rna Silencing Technology to Mitigate the Voriconazole Resistance of Aspergillus Flavus; Lipofectamine-Based Delivery, Advanced Pharmaceutical Bulletin (2017)
8. Effect of Involved Aspergillus Species on Galactomannan in Bronchoalveolar Lavage of Patients With Invasive Aspergillosis, Journal of Medical Microbiology (2017)
Experts (# of related papers)
View all Related Experts
Sassan Rezaie (3)
Sadegh Khodavaisy (3)