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Two- and Three-Way Chemometric Analyses for Investigation of Interactions of Acarbose With Normal and Glycated Human Serum Albumin: Developing a Novel Biosensing System Publisher



Nazari M1, 2 ; Kashanian S3 ; Omidfar K4 ; Ghobadi S5 ; Goicoechea HC6 ; Gu HW7 ; Khodarahmi R8 ; Jalalvand AR9
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Authors Affiliations
  1. 1. Faculty of Chemistry, Razi University, Kermanshah, Iran
  2. 2. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran
  4. 4. Endocrine and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, P.O. Box 14395/1179, Tehran, Iran
  5. 5. Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
  6. 6. Laboratorio de Desarrollo Analitico y Quimiometria (LADAQ), Catedra de Quimica Analitica I, Universidad Nacional del Litoral, Ciudad Universitaria, CC 242, S3000ZAA Santa Fe, Argentina
  7. 7. College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, 434023, China
  8. 8. Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
  9. 9. Research Center of Oils and Fats, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah, Iran

Source: Microchemical Journal Published:2021


Abstract

In this project, our research group has performed an interesting work in which interactions of acarbose (ACB) with normal human serum albumin (HSA) and glycated HSA (GHSA) have been investigated by chemometrics assisted-electrochemical and spectroscopic techniques. To have a deep insight to the interactions of ACB with HSA and GHSA, different electrochemical and spectroscopic techniques were used to monitor ACB-HSA and ACB-GHSA interactions and analyzed by multivariate curve resolution alternating least squares (MCR-ALS), MCR-BANNDS and EQUISPEC as efficient chemometric algorithms. Then, molecular docking techniques were used to extract more information which helped us to better justify binding of ACB with HSA and GHSA. After obtaining qualitative and quantitative information and justification of the ACB-HSA and ACB-GHSA interactions, a novel electrochemical technique was developed for exploiting second-order advantage from differential pulse voltammetric data for determination of GHSA as a potential biomarker in diabetes in the presence of HSA. Calibration and validation of the developed system showed us that our system was successful in determination of GHSA in synthetic and real samples. © 2020 Elsevier B.V.