Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro

Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro Publisher Pubmed

Tari K¹ ; Atashi A² ; Kaviani S¹ ; Akhavanrahnama M³ ; Anbarlou A³ ; Mossahebimohammadi M¹

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1. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2. Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
3. Department of Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Biologicals Published:2017

Abstract

Background Hepatocyte Growth Factor (HGF) plays a pivotal role in hematopoiesis, motility, growth and mobilization of hematopoietic stem/progenitor cells (HSPCs). HGF mainly is produced by bone marrow mesenchymal stem cells (BM-MSCs). MSCs express erythropoietin (EPO) receptor. In this study, we aimed to assess the effect of EPO on HGF secretion in BM-MSCs. Methods The BM-MSCs treated with EPO (4 IU/ml) for 6, 24 and 48 h. HGF gene expression and protein level were assessed using quantitative real time PCR (qRT-PCR) and Enzyme-linked immunosorbant Assay. In order to show the effect of secreted HGF on migration of HSPCs, hematopoietic stem cells (HSCs) were isolated from cord blood and evaluated using transwell migration assay. Results We observed a significant increase in level of HGF in cell supernatant after 48 h compared to control group (P < 0.05). Also, qRT-PCR results demonstrated a significant elevation in HGF expression level after 24 and 48 h treatment with EPO compared to control group (P < 0.05). Finally, migration assay results showed a significant increase in migration of HSCs in treated group after 48 h. Conclusion Our data indicated that EPO may play an important role in stem cell mobilization through up regulating HGF in MSCs and inducing migration of HSCs. © 2016 International Alliance for Biological Standardization

Style	Citing Format
MLA	Tari K, et al.. "Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro." Biologicals, vol. 45, no. , 2017, pp. 15-19.
APA	Tari K, Atashi A, Kaviani S, Akhavanrahnama M, Anbarlou A, Mossahebimohammadi M (2017). Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro. Biologicals, 45(), 15-19.
Chicago	Tari K, Atashi A, Kaviani S, Akhavanrahnama M, Anbarlou A, Mossahebimohammadi M. "Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro." Biologicals 45, no. (2017): 15-19.
Harvard	Tari K et al. (2017) 'Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro', Biologicals, 45(), pp. 15-19.
Vancouver	Tari K, Atashi A, Kaviani S, Akhavanrahnama M, Anbarlou A, Mossahebimohammadi M. Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro. Biologicals. 2017;45():15-19.
BibTex	@article{ author = {Tari K and Atashi A and Kaviani S and Akhavanrahnama M and Anbarlou A and Mossahebimohammadi M}, title = {Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro}, journal = {Biologicals}, volume = {45}, number = {}, pages = {15-19}, year = {2017} }
RIS	TY - JOUR AU - Tari K AU - Atashi A AU - Kaviani S AU - Akhavanrahnama M AU - Anbarlou A AU - Mossahebimohammadi M TI - Erythropoietin Induces Production of Hepatocyte Growth Factor From Bone Marrow Mesenchymal Stem Cells In Vitro JO - Biologicals VL - 45 IS - SP - 15 EP - 19 PY - 2017 ER -

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