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Effect of Neohesperidin Dihydrochalcone on the Activity and Stability of Alpha-Amylase: A Comparative Study on Bacterial, Fungal, and Mammalian Enzymes Publisher Pubmed



Kashaniamin E1, 2 ; Ebrahimhabibi A1, 2 ; Larijani B2 ; Moosavimovahedi AA3
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Authors Affiliations
  1. 1. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Shariati Hospital, North Kargar Avenue, Tehran, 1411413137, Iran
  2. 2. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

Source: Journal of Molecular Recognition Published:2015


Abstract

Neohesperidin dihydrochalcone (NHDC) was recently introduced as an activator of mammalian alpha-amylase. In the current study, the effect of NHDC has been investigated on bacterial and fungal alpha-amylases. Enzyme assays and kinetic analysis demonstrated the capability of NHDC to significantly activate both tested alpha-amylases. The ligand activation pattern was found to be more similar between the fungal and mammalian enzyme in comparison with the bacterial one. Further, thermostability experiments indicated a stability increase in the presence of NHDC for the bacterial enzyme. In silico (docking) test locates a putative binding site for NHDC on alpha-amylase surface in domain B. This domain shows differences in various alpha-amylase types, and the different behavior of the ligand toward the studied enzymes may be attributed to this fact. Copyright © 2015 John Wiley & Sons, Ltd.
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Azadeh Ebrahim-Habibi (7)
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