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Comparing the Effect of Plasma Therapy With Estradiol Valerate in Motor and Cognitive Behavioral Disorders in Ovariectomized Old Rats: Behavioral, Biochemical, and Protein Expression Publisher



Khajvandabedini M1 ; Mohammadi M2 ; Habibi P3 ; Shahabi Z4 ; Shahidi S4 ; Ahmadiasl N5 ; Alipour MR4 ; Ramezani M6 ; Komaki A4
Authors
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Authors Affiliations
  1. 1. Department of Clinical Biochemistry, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Department of Medical Laboratory Science, Faculty of Medicine, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
  3. 3. Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  5. 5. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Source: Iranian Journal of Basic Medical Sciences Published:2025


Abstract

Objective(s): This study investigated the effects of young plasma therapy (YPT) compared to estrogen therapy (E2T) on motor and cognitive impairments in aged ovariectomized (OVX) rats. Materials and Methods: Sixty female Wistar rats were divided as follows: 1). 2-3 months control young group. Five 22-24 months old groups: 1) Control, 2) Sham, 3) OVX, 4) OVX.E2, and 5) OVX. YP. Young plasma (1 ml plasma, through the tail vein, 3 days weekly for 4 weeks) and E2 (30 mg/ kg, SC, 5 days weekly for 4 weeks) were administrated to OVX rats. The open field, elevated plus maze, and Barne's maze were used to assess the behaviors. Then, miR-134 and miR-124 (RT- RCR), SIRT1, CREB, and BDNF (western blot), and anti-oxidants/oxidants markers (Photometry) levels were assessed in the rat's hippocampal tissues. Results: OVX caused up-regulated hippocampal miR-134 and miR-124 expression levels (P<0.001) while down-regulated SIRT1, CREB, and BDNF protein expressions (P<0.001). Also, ovariectomy Increased TOS, OSI, and MDA (P<0.001) while decreasing TAC (P<0.001) compared to sham. Treatment with both E2T and YPT significantly improved all oxidative stress indexes (P<0.0.001) and increased p-CREB, BDNF, and SIRT1 protein levels (P<0.05, P<0.01) while decreasing the expression of miR-134 and miR-124 (P<0.001). Conclusion: YPT is a non-pharmacological therapeutic as much as or more than E-2T, which can exhibit anti-oxidative and anti-inflammatory potential in the hippocampal tissue and improve cognitive deficits in aged OVX rats without unknown side effects. © 2025 Mashhad University of Medical Sciences. All rights reserved.