Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Detection of Toxoplasma-Specific Immunoglobulin G in Human Sera: Performance Comparison of in House Dot-Elisa With Eclia and Elisa Publisher Pubmed



Teimouri A1, 2 ; Modarressi MH3 ; Shojaee S1 ; Mohebali M1 ; Zouei N1 ; Rezaian M1 ; Keshavarz H1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Pour Sina Street, Ghods Avenue, Enghelab Avenue, P.O. Box: 1417613191, Tehran, Iran
  2. 2. Students Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: European Journal of Clinical Microbiology and Infectious Diseases Published:2018


Abstract

In the current study, performance of electrochemiluminescence immunoassay (ECLIA) in detection of anti-toxoplasma IgG in human sera was compared with that of enzyme-linked immunosorbent assay (ELISA). Furthermore, performance of an in house Dot-ELISA in detection of anti-toxoplasma IgG was compared with that of ECLIA and ELISA. In total, 219 human sera were tested to detect anti-toxoplasma IgG using Dynex DS2® and Roche Cobas® e411 Automated Analyzers. Discordant results rechecked using immunofluorescence assay (IFA). Then, sera were used in an in house Dot-ELISA to assess toxoplasma-specific IgG. Of the 219 samples, two samples were found undetermined using ECLIA but reactive using ELISA. Using IFA, the two sera were reported unreactive. Furthermore, two samples were found reactive using ECLIA and unreactive using ELISA. These samples were reported reactive using IFA. The overall agreement for the two former methods was 98% (rZ0.98.1; P < 0.001). The intrinsic parameters calculated for in house Dot-ELISA included sensitivity of 79.5, specificity of 78.2, and accuracy of 78.9%, compared to ECLIA and ELISA. Positive and negative predictive values included 82.9 and 74.2%, respectively. A 100% sensitivity was found in in house Dot-ELISA for highly reactive sera in ECLIA and ELISA. ECLIA is appropriate for the first-line serological screening tests and can replace ELISA due to high speed, sensitivity, and specificity, particularly in large laboratories. Dot-ELISA is a rapid, sensitive, specific, cost-effective, user-friendly, and field-portable technique and hence can be used for screening toxoplasmosis, especially in rural fields or less equipped laboratories. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
Related Docs
View other Related Docs
1. Development, Optimization, and Validation of an In-House Dot-Elisa Rapid Test Based on Sag1 and Gra7 Proteins for Serological Detection of Toxoplasma Gondii Infections, Infection and Drug Resistance (2019)
2. Role of Toxoplasma Gondii Igg Avidity Testing in Discriminating Between Acute and Chronic Toxoplasmosis in Pregnancy, Journal of Clinical Microbiology (2020)
3. Early Detection of Toxoplasma Gondii Infection by Using a Interferon Gamma Release Assay: A Review, Experimental Parasitology (2017)
4. Assessment of an In-House Enzyme-Linked Immunosorbent Assay and Igg Avidity Test Based on Sag1 and Gra7 Proteins for Discriminating Between Acute and Chronic Toxoplasmosis in Humans, Journal of Clinical Microbiology (2021)
5. Seroepidemiological Study of Toxoplasmosis in Women Referred to a Pre-Marriage Counseling Center in Alborz Province, Iran, BMC Research Notes (2021)
6. Detection of Toxoplasma Gondi in Acute and Chronic Phases of Infection in Immunocompromised Patients and Pregnant Women With Real-Time Pcr Assay Using Taqman Fluorescent Probe, Iranian Journal of Parasitology (2018)
7. Utility of Blood As the Clinical Specimen for the Diagnosis of Ocular Toxoplasmosis Using Uracil Dna Glycosylase-Supplemented Loop-Mediated Isothermal Amplification and Real-Time Polymerase Chain Reaction Assays Based on Rep-529 Sequence and B1 Gene, BMC Infectious Diseases (2022)
8. The Relation of Serum Prolactin Levels and Toxoplasma Infection in Humans, International Journal of General Medicine (2019)
Experts (# of related papers)
View all Related Experts
Hossein Keshavarz Valian (21)
Saeedeh Shojaee (19)
Other Related Docs
9. Toxoplasma Gondii Infection in Immunocompromised Patients in Iran (2013-2022): A Systematic Review and Meta-Analysis, Iranian Journal of Parasitology (2022)
10. Anti-Toxoplasma Activity of Various Molecular Weights and Concentrations of Chitosan Nanoparticles on Tachyzoites of Rh Strain, International Journal of Nanomedicine (2018)
11. Design of a Dual-Promoter Expression Vector Harboring Sag1 and Gra7 Genes From Toxoplasma Gondii (Rh Strain), Tropical Biomedicine (2018)
12. The Relation of Secondary Sex Ratio and Miscarriage History With Toxoplasma Gondii Infection, BMC Infectious Diseases (2018)
13. Development of High Resolution Melting Analysis As a Diagnostic Tool for Molecular Detection of Toxoplasma Infection in Pregnant Women and Hiv Positive Cases, Iranian Journal of Public Health (2020)
14. Prevalence and Predictors of Toxoplasma Gondii Infection in Psychiatric Inpatients in Fars Province, Southern Iran, Frontiers in Psychiatry (2022)
15. Curcumin Nanoemulsion As a Novel Chemical for the Treatment of Acute and Chronic Toxoplasmosis in Mice, International Journal of Nanomedicine (2018)
16. The Association of Latent Toxoplasmosis and Level of Serum Testosterone in Humans, BMC Research Notes (2018)
17. Molecular Detection of Toxoplasma Gondii Oocytes in Soil Samples From Guilan Province, Northern Iran, Iranian Journal of Public Health (2024)
18. In Vitro Culture of Toxoplasma Gondii in Hela, Vero, Rbk and A549 Cell Lines, Infezioni in Medicina (2018)
19. Detection and Genotyping of Toxoplasma Gondii Isolated From Soil in Ahvaz, Southwest of Iran, Journal of Parasitic Diseases (2017)
20. Immunodiagnosis and Molecular Validation of Toxoplasma Gondii Infection Among Patients With End-Stage Renal Disease Undergoing Haemodialysis, Parasitology (2019)
21. Toward Waterborne Protozoa Detection Using Sensing Technologies, Frontiers in Microbiology (2023)
22. Seroprevalence and Risk Factors Associated With Toxoplasma Gondii Infection Among Rural Communities in Northern Iran, Revista do Instituto de Medicina Tropical de Sao Paulo (2016)
23. Diagnosis of Toxoplasma Infection in Allogenic Pre Hctsp Patients Using Molecular Methods, Iranian Journal of Parasitology (2022)
24. Comparison of the Toxoplasma Gondii Mice and Cell Culture Derived Antigens in Elisa Assay, Tropical Biomedicine (2017)
25. Nanoemulsion of Spiramycin Against Tachyzoites of Toxoplasma Gondii, Rh Strain: Preparation, Toxicology, and Efficacy Studies, Iranian Journal of Public Health (2023)
26. Detection of Toxoplasma Gondii in Cord Blood Samples From Neonates in Tehran, Iran, Iranian Journal of Public Health (2019)
27. Frequency of Sero-Positivity in Household Members of the Patients With Positive Toxoplasma Serology; [Frecuencia De Seropositividad En Los Miembros De Hogares De Pacientes Con Serologia Positiva Para Toxoplasma], Revista Espanola de Quimioterapia (2018)
28. Serological Survey of Neospora Caninum and Toxoplasma Gondii Co-Infection in Rodents in Northwestern Iran, Iranian Journal of Parasitology (2020)
29. Associations Between Toxoplasma Gondii Infection and Multiple Sclerosis: A Case-Control Seroprevalence Study, Iranian Journal of Parasitology (2023)
30. Assessment of the Immunogenicity and Protective Efficiency of a Novel Dual-Promoter Dna Vaccine, Harboring Sag1 and Gra7 Genes, From Rh Strain of Toxoplasma Gondii in Balb/C Mice, Infection and Drug Resistance (2019)