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Evaluation of Structure, Chaperone-Like Activity and Allergenicity of Reduced Glycated Adduct of Bovine Β-Casein Publisher Pubmed



Yousefi R1 ; Ferdowsi L1 ; Tavaf Z1 ; Sadeghian T1 ; Tamaddon AM2 ; Moghtaderi M3 ; Pourpak Z4
Authors
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Authors Affiliations
  1. 1. Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
  2. 2. Center for Nanotechnology in Drug Delivery, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
  3. 3. Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  4. 4. Immunology, Asthma, and Allergy Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Protein and Peptide Letters Published:2017


Abstract

Milk has a potent reducing environment with an important quantity of sugar levels. In the current study β-casein was glycated in the presence of D-glucose and sodium cyanoborohydride as a reducing agent. Then, the reduced glucitol adduct of β-casein was used for the structural and functional analyses using different spectroscopic techniques. The results of fluorescence and far ultraviolet circular dichroism assessments suggest important structural alteration upon non-enzymatic glycation of β-casein. In addition, the chaperone activity, micellization properties and antioxidant activity of this protein were altered upon glucose modification. Also, as a result of reduced glycation, the allergenicity profile of this protein remained largely unchanged. Additional to its energetic and nutritional values, β-casein has important functional properties. The native structure of this protein is important to perform accurately its biological functions. Non-enzymatic glycation under reducing state was capable to alter both structural and functional aspects of β-casein. Due to effective reducing environment and significant quantity of reducing sugar of human milk, similar structural and functional alterations are most likely to occur upon reducing glycation of β-casein in vivo. Also, these changes might be even intensified during chronic hyperglycemia in diabetic mothers. © 2017 Bentham Science Publishers.