Yeast Expression Systems: Overview and Recent Advances

Yeast Expression Systems: Overview and Recent Advances Publisher Pubmed

Baghban R^{1, 2, 3} ; Farajnia S^{3, 4} ; Rajabibazl M^{5, 6} ; Ghasemi Y⁷ ; Mafi AA⁸ ; Hoseinpoor R⁶ ; Rahbarnia L⁹ ; Aria M³

Show Affiliations

1. Medical Biotechnology Department, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran
2. Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3. Biotechnology Research Center, Tabriz University of Medical Sciences, Daneshgah Ave, Tabriz, Iran
4. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5. Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Velenjak, Arabi Ave, Tehran, Iran
6. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Shiraz University of Medical Science, Shiraz, Iran
8. Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
9. Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Molecular Biotechnology Published:2019

Abstract

Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been emerged on yeast due to the inherent advantages and new developments in this host cell. For the production of each specific product, the most appropriate expression system should be identified and optimized both on the genetic and fermentation levels, considering the features of the host, vector and expression strategies. Currently, several new systems are commercially available; some of them are private and need licensing. The potential for secretory expression of heterologous proteins in yeast proposed this system as a candidate for the production of complex eukaryotic proteins. The common yeast expression hosts used for recombinant proteins’ expression include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis, and Schizosaccharomyces pombe. This review is dedicated to discuss on significant characteristics of the most common methylotrophic and non-methylotrophic yeast expression systems with an emphasis on their advantages and new developments. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.

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Style	Citing Format
MLA	Baghban R, et al.. "Yeast Expression Systems: Overview and Recent Advances." Molecular Biotechnology, vol. 61, no. 5, 2019, pp. 365-384.
APA	Baghban R, Farajnia S, Rajabibazl M, Ghasemi Y, Mafi AA, Hoseinpoor R, Rahbarnia L, Aria M (2019). Yeast Expression Systems: Overview and Recent Advances. Molecular Biotechnology, 61(5), 365-384.
Chicago	Baghban R, Farajnia S, Rajabibazl M, Ghasemi Y, Mafi AA, Hoseinpoor R, Rahbarnia L, Aria M. "Yeast Expression Systems: Overview and Recent Advances." Molecular Biotechnology 61, no. 5 (2019): 365-384.
Harvard	Baghban R et al. (2019) 'Yeast Expression Systems: Overview and Recent Advances', Molecular Biotechnology, 61(5), pp. 365-384.
Vancouver	Baghban R, Farajnia S, Rajabibazl M, Ghasemi Y, Mafi AA, Hoseinpoor R, et al.. Yeast Expression Systems: Overview and Recent Advances. Molecular Biotechnology. 2019;61(5):365-384.
BibTex	@article{ author = {Baghban R and Farajnia S and Rajabibazl M and Ghasemi Y and Mafi AA and Hoseinpoor R and Rahbarnia L and Aria M}, title = {Yeast Expression Systems: Overview and Recent Advances}, journal = {Molecular Biotechnology}, volume = {61}, number = {5}, pages = {365-384}, year = {2019} }
RIS	TY - JOUR AU - Baghban R AU - Farajnia S AU - Rajabibazl M AU - Ghasemi Y AU - Mafi AA AU - Hoseinpoor R AU - Rahbarnia L AU - Aria M TI - Yeast Expression Systems: Overview and Recent Advances JO - Molecular Biotechnology VL - 61 IS - 5 SP - 365 EP - 384 PY - 2019 ER -

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Abstract