Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field

Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field Publisher Pubmed

Mirahmadi H^{1, 2} ; Kazemipour N³ ; Yazdiani A³ ; Mehravaran A^{1, 2} ; Basseri HR⁴ ; Mohammadi L¹ ; Alijani E⁵

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1. Infectious Disease and Tropical Medicine Research Center, Resistance Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran
2. Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
3. Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Kerman, Iran
4. Department of Medical Entomology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
5. Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran

Source: Ethiopian journal of health sciences Published:2021

Abstract

Background: Malaria is one of the main parasitic diseases and a major health issue in some countries. This study aims to determine the rate and type of infections of Anopheles mosquitoes with malaria parasites using the molecular LAMP method in the Southeastern Iran. Methods: In this study, 400 Anopheles mosquitoes were collected by the Zahedan Medical Insecticide Center in Nikshahr City, a high-risk area of malaria transmission in Sistan-Baluchestan Province. The mosquitoes were caught manually (by hand) in domestic (humans and animals), natural, and artificial outdoor places (Shelter pits). After DNA extraction, the LAMP method was used, which was compared with Multiplex Nested-PCR as a standard method. Results: Out of 400 samples collected from Nikshahr City, 6 samples (1.5%) were infected with Plasmodium vivax. No Plasmodium falciparum or a mix (Plasmodium vivax and Plasmodium falciparum) was detected in this study. Conclusions: The results of this study indicate that in places with transmission of both species, i.e. Plasmodium vivax and Plasmodium falciparum, detection of malaria parasites by the LAMP method could be very useful in spotting infections in the field. Thus, molecular epidemiological studies could be conducted annually to monitor malaria in endemic regions. The results of this research show that contamination with mosquito malaria vectors is increasing in Nikshahr City, and it seems that more studies will be required to eliminate malaria until 2026. © 2021 Hadi M., etal.

Style	Citing Format
MLA	Mirahmadi H, et al.. "Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field." Ethiopian journal of health sciences, vol. 31, no. 4, 2021, pp. 743-752.
APA	Mirahmadi H, Kazemipour N, Yazdiani A, Mehravaran A, Basseri HR, Mohammadi L, Alijani E (2021). Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field. Ethiopian journal of health sciences, 31(4), 743-752.
Chicago	Mirahmadi H, Kazemipour N, Yazdiani A, Mehravaran A, Basseri HR, Mohammadi L, Alijani E. "Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field." Ethiopian journal of health sciences 31, no. 4 (2021): 743-752.
Harvard	Mirahmadi H et al. (2021) 'Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field', Ethiopian journal of health sciences, 31(4), pp. 743-752.
Vancouver	Mirahmadi H, Kazemipour N, Yazdiani A, Mehravaran A, Basseri HR, Mohammadi L, et al.. Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field. Ethiopian journal of health sciences. 2021;31(4):743-752.
BibTex	@article{ author = {Mirahmadi H and Kazemipour N and Yazdiani A and Mehravaran A and Basseri HR and Mohammadi L and Alijani E}, title = {Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field}, journal = {Ethiopian journal of health sciences}, volume = {31}, number = {4}, pages = {743-752}, year = {2021} }
RIS	TY - JOUR AU - Mirahmadi H AU - Kazemipour N AU - Yazdiani A AU - Mehravaran A AU - Basseri HR AU - Mohammadi L AU - Alijani E TI - Investigation of Lamp Technique in Diagnosis Type of Plasmodium Species in Anopheles Mosquitoes :A Fast and Practical Technique to Detect Malaria Pathogens in the Field JO - Ethiopian journal of health sciences VL - 31 IS - 4 SP - 743 EP - 752 PY - 2021 ER -

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