Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity

Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity Publisher Pubmed

Rahimi A¹ ; Hosseinnataj H¹ ; Hajheydari Z² ; Aryanian Z^{3, 4} ; Shayannia A⁵ ; Ajami A¹ ; Asgarianomran H^{1, 6}

Show Affiliations

1. Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
2. Department of Dermatology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
3. Department of Dermatology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
4. Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
6. Gastrointestinal Cancer Research Center, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran

Source: Experimental Dermatology Published:2019

Abstract

The contribution of immune checkpoint receptors in the immunopathogenesis of various autoimmune diseases has been addressed in previous reports. In this study, the expression profile of T-cell immunoglobulin and mucin-domain containing-3 (Tim-3) and programmed cell death-1 (PD-1) checkpoint molecules was investigated in CD8+ T cells of Vitiligo patients. The association of Tim-3 and PD-1 expression with disease activity was also explored. The frequency of Tim-3+/PD-1+/CD8+ T cells in 30 patients with vitiligo and 30 sex- and age-matched controls was determined by flow cytometry. CD8+ T cells were then positively isolated by magnetic beads, and the mRNA expression of PD-1 and Tim-3 was determined by TaqMan-based real-time PCR. To measure the cytokines production, PBMCs were stimulated with PMA/ionomycin and concentrations of IL-4, IFN-γ and TNF-α were measured in culture supernatants by ELISA. Disease activity of patients with vitiligo was determined using the Vitiligo Area Severity Index. Patients with vitiligo have significantly shown more expression of Tim-3 and PD-1 on their CD8+ T cells compared with controls. Expression analysis of Tim-3 mRNA, but not PD-1, confirmed the results obtained from flow cytometry. While the production levels of TNF-α and IFN-γ were found higher by patients with vitiligo, IL-4 production was lower in patients compared with controls. A direct association was observed between the Tim-3 and PD-1 expression and also the production of pro-inflammatory cytokines with disease activity of patients with vitiligo. Our results indicate that Tim-3 and PD-1 are involved in immune dysregulation mechanisms of CD8+ T cells in vitiligo and may introduce as potential biomarkers for disease progression and targeted immunotherapy. © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Style	Citing Format
MLA	Rahimi A, et al.. "Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity." Experimental Dermatology, vol. 28, no. 6, 2019, pp. 674-681.
APA	Rahimi A, Hosseinnataj H, Hajheydari Z, Aryanian Z, Shayannia A, Ajami A, Asgarianomran H (2019). Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity. Experimental Dermatology, 28(6), 674-681.
Chicago	Rahimi A, Hosseinnataj H, Hajheydari Z, Aryanian Z, Shayannia A, Ajami A, Asgarianomran H. "Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity." Experimental Dermatology 28, no. 6 (2019): 674-681.
Harvard	Rahimi A et al. (2019) 'Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity', Experimental Dermatology, 28(6), pp. 674-681.
Vancouver	Rahimi A, Hosseinnataj H, Hajheydari Z, Aryanian Z, Shayannia A, Ajami A, et al.. Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity. Experimental Dermatology. 2019;28(6):674-681.
BibTex	@article{ author = {Rahimi A and Hosseinnataj H and Hajheydari Z and Aryanian Z and Shayannia A and Ajami A and Asgarianomran H}, title = {Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity}, journal = {Experimental Dermatology}, volume = {28}, number = {6}, pages = {674-681}, year = {2019} }
RIS	TY - JOUR AU - Rahimi A AU - Hosseinnataj H AU - Hajheydari Z AU - Aryanian Z AU - Shayannia A AU - Ajami A AU - Asgarianomran H TI - Expression Analysis of Pd-1 and Tim-3 Immune Checkpoint Receptors in Patients With Vitiligo; Positive Association With Disease Activity JO - Experimental Dermatology VL - 28 IS - 6 SP - 674 EP - 681 PY - 2019 ER -

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