Tyrosinase Inhibition and Angiogenesis Studies of Zinc Oxide Nanoparticles Green Synthesized Using Aqueous Extract of Peucedanum Knappii Publisher



Sarkhail P ; Foroughian S ; Gholami M ; Navidpour L
Source: Nanomedicine Research Journal Published:2025

Abstract

To investigate the anti-tyrosinase and angiogenic properties of zinc oxide nanoparticles (ZONPs), an aqueous extract prepared from the aerial parts of Peucedanum knappii Bornm., known to be rich in glycosylated phenolics and polyphenolics, was employed for the green synthesis of ZONPs. The ZONPs were produced by adding a Zn salt solution to the aqueous of the plant. High–performance liquid chromatography (HPLC) was employed to examine chromatographic changes in the extract before and after nanoparticle formation. The synthesized ZONPs were comprehensively characterized using UV–Visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and X–ray diffraction (XRD). The effect of ZONPs on tyrosinase enzyme activity was assessed through an in vitro mushroom tyrosinase inhibition assay, whereas their angiogenic potential was evaluated in vivo using the chicken chorioallantoic membrane (CAM) assay. The results confirmed the successful green synthesis of ZONPs utilizing P. knappii aqueous extract (PAE). HPLC analysis indicated that multiple phytochemicals present in PAE contributed to nanoparticle formation. Characterization data revealed that the ZONPs exhibited a hexagonal crystalline ZnO structure with an average crystallite size of 19.98 nm. The nanoparticles showed notable anti-tyrosinase activity, with an IC50 value of 143.28 μg/ml, and promoted angiogenesis in the CAM assay at concentrations between 125 and 250 μg/ml without inducing toxicity. Overall, these findings demonstrate that PAE acts as an effective reducing, capping, and stabilizing agent in the green synthesis of ZONPs, which possess promising biological properties for potential applications in skin-whitening cosmetics and wound-healing formulations. © 2025 Tehran University of Medical Sciences. All rights reserved.