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Infrared Microspectroscopy Studies on the Protective Effect of Curcumin Coated Gold Nanoparticles Against H2o2-Induced Oxidative Stress in Human Neuroblastoma Sk-N-Sh Cells Publisher Pubmed



Karimi F1 ; Shaabani E1 ; Martinezrovira I2, 3 ; Yousef I2 ; Ghahremani MH4 ; Kharrazi S1
Authors
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Authors Affiliations
  1. 1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences (TUMS), Tehran, Iran
  2. 2. ALBA-CELLS Synchrotron, MIRAS Beamline, Carrer de la Llum 2-26, Cerdanyola del Valles, 09290, Spain
  3. 3. Ionizing Radiation Research Group (GRRI), Physics Department, Universitat Autonoma de Barcelona (UAB), Edifici C. Campus de la UAB, Avinguda de l'Eix Central, Cerdanyola del Valles, 08193, Spain
  4. 4. Department of Toxicology-Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Analyst Published:2021


Abstract

The contribution of oxidative stress in several chronic and degenerative diseases suggests that antioxidant therapy can be a promising therapeutic strategy. However, in the case of many antioxidants, their biodistribution and bioactivity are restricted due to low water solubility. Curcumin is a powerful free radical scavenger that upon conjugation to gold nanoparticles results in the formation of stable gold nanoparticles that act as highly water-soluble carriers for the curcumin molecules. In the present study, the effect of curcumin-coated gold nanoparticles (Cur-GNPs) on the H2O2-treated human neuroblastoma (SK-N-SH) cell line was evaluated by using Fourier transform infrared (FTIR) microspectroscopy. Biochemical changes in cells resulting from exposure to reactive oxygen species (ROS) and antioxidant treatment on cells were investigated. Analyzing changes in PO2- bands and amide bands in the fingerprint region and also changes in the ratio of CH2(asym) to CH3(asym) bands in the lipid region revealed that post-treatment with Cur-GNPs could effectively decrease the damage on DNA caused by H2O2 treatment, whereas pre-treatment of cells with Cur-GNPs was found to be more effective at preventing lipid peroxidation than post-treatment. Further analysis of the CH2(asym) to CH3(asym) ratio provided information on not only the lipid peroxidation level in cells, but also the interaction of nanoparticles with the plasma membrane, as confirmed by lactate dehydrogenase assay. This journal is © The Royal Society of Chemistry.