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Static Magnetic Field Effects on the Secondary Structure and Elasticity of Collagen Molecules; a Possible Biophysical Approach to Treat Keratoconus Publisher Pubmed



Akbari M1 ; Mobasheri H1, 2 ; Noorizadeh F3 ; Daryabari SH4 ; Dini L5
Authors
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Authors Affiliations
  1. 1. Laboratory of Membrane Biophysics and Macromolecules, Institute of Biochemistry and Biophysics, University of Tehran, Iran
  2. 2. Institute of Biomaterials of University of Tehran and Tehran University of Medical Sciences (IBUTUMS), Tehran, Iran
  3. 3. Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Basir Eye Health Research Center and Baqiyatallah University of Medical Sciences, Tehran, Iran
  5. 5. Department of Biology and Biotechnology C. Darwin, Sapienza University of Rome, Rome, Italy

Source: Biochemical and Biophysical Research Communications Published:2024


Abstract

Type I collagen is among the major extracellular proteins that play a significant role in the maintenance of the cornea's structural integrity and is essential in cell adhesion, differentiation, growth, and integrity. Here, we investigated the effect of 300 mT Static Magnetic Field (300 mT SMF) on the structure and molecular properties of acid-solubilized collagens (ASC) isolated from the rat tail tendon. The SMF effects at molecular and atomic levels were investigated by various biophysical approaches like Circular Dichroism Spectropolarimetery (CD), Fourier Transform Infrared Spectroscopy (FTIR), Zetasizer light Scattering, and Rheological assay. Exposure of isolated type I collagen to 300 mT SMF retained its triple helix. The elasticity of collagen molecules and the keratoconus (KCN) cornea treated with SMF decreased significantly after 5 min and slightly after 10, 15, and 20 min of treatments. The exposure to 300 mT SMF shifted the Amid I bond random coil to antiparallel wave number from 1647 to 1631 cm−1. The pH of the 300 mT SMF treated collagen solution increased by about 25 %. The treatment of the KCN corneas with 300 mT SMF decreased their elasticity significantly. The promising results of the effects of 300 mT SMF on the collagen molecules and KCN cornea propose a novel biophysical approach capable of manipulating the collagen's elasticity, surface charges, electrostatic interactions, cross binding, network formation and fine structure. Therefore, SMF treatment may be considered as a novel non-invasive, direct, non-chemical and fast therapeutic and manipulative means to treat KCN cornea where the deviated physico-chemical status of collagen molecules cause deformation. © 2024 Elsevier Inc.
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